Invitrogen

PARK7 Monoclonal Antibody (GT136)

查看其他30个DJ-1抗体

问题与答案

问题与答案

抗体检测数据 (5)
高级验证 (1)

PARK7 Antibody in Immunocytochemistry (ICC/IF)

PARK7 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

PARK7 Antibody (MA5-38261) in ICC/IF

Immunocytochemistry analysis of PARK7 in 4% paraformaldehyde (RT, 15 min) fixed DIV9 rat E18 primary hippocampal neuron cells (Green: Park7 / DJ-1, Red: Tau). Samples was incubated in PARK7 monoclonal antibody (Product # MA5-38261) using a dilution of 1:500, followed by DAPI.

PARK7 Antibody in Immunocytochemistry (ICC/IF)

PARK7 Antibody in Western Blot (WB)

PARK7 Antibody in Western Blot (WB)

PARK7 Antibody in Western Blot (WB)

PARK7 Antibody in Western Blot (WB)

Product Image Gallery

PARK7 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of PARK7 in 4% paraformaldehyde (RT, 15 min) fixed DIV9 rat E18 primary hippocampal neuron cells (Green: Park7 / DJ-1, Red: Tau). Samples was incubated in PARK7 monoclonal antibody (Product # MA5-38261) using a dilution of 1:500, followed by DAPI.

PARK7 Antibody in Western Blot (WB) — Knockout of PARK7 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR634073_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of PARK7 was performed by loading 30 µg of HeLa wild type (Lane 1), HeLa Cas9 (Lane 2) and HeLa PARK7 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with PARK7 Monoclonal Antibody (GT136) (Product # MA5-38261, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:10,000 dilution). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076) with the iBright™ FL1500 (Product # A44115). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to PARK7.

PARK7 Antibody in Western Blot (WB) — Western blot analysis of PARK7 in 293T whole cell extracts (30 µg). Samples were incubated with PARK7 monoclonal antibody (Product # MA5-38261) using a dilution of 1:10,000 followed by HRP-conjugated anti-mouse IgG. Separated by 15% SDS-PAGE.

PARK7 Antibody in Western Blot (WB) — Western blot analysis of PARK7 in Mouse tissue extract (50 µg). Samples were separated with 15% SDS-PAGE and incubated in PARK7 monoclonal antibody (Product # MA5-38261) using a dilution of 1:1,000, followed by HRP-conjugated anti-mouse IgG. Detection was performed with Trident ECL plus-Enhanced.

PARK7 Antibody in Western Blot (WB) — Western blot analysis of PARK7 in Various whole cell extracts (30 µg). Samples were separated with 12% SDS-PAGE and incubated in PARK7 monoclonal antibody (Product # MA5-38261) using a dilution of 1:1,000, followed by HRP-conjugated anti-mouse IgG.

PARK7 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inPARK7 KO cell line compared to control cell line using PARK7 Monoclonal Antibody (GT136) (Product # MA5-38261). {KO}

Immunogen image

PARK7 Monoclonal Antibody (GT136)

Immunogen Image PARK7 Monoclonal Antibody (GT136)

产品信息

MA5-38261

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5000-1:20000

-

免疫细胞化学 (ICC/IF)

1:100-1:1,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

GT136

抗原

Full length human Park7 / DJ-1 recombinant protein.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Centrifuge briefly prior to opening.

靶标信息

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Contraception-associated protein 1; CTA-215D11.1; DJ-1; early onset 7; epididymis secretory sperm binding protein Li 67p; Fertility protein SP22; FLJ27376; FLJ34360; FLJ92274; Maillard deglycase; Oncogene DJ1; OTTHUMP00000001350; OTTHUMP00000001351; PARK7 GENE; PARK7/DJ1; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; Parkinson disease protein 7 homolog; parkinson protein 7; Parkinsonism-associated deglycase; Protein CAP1; protein deglycase DJ-1; Protein DJ-1; Protein/nucleic acid deglycase DJ-1; RNA-binding protein regulatory subunit

查看更多收起

基因别名: CAP1; DJ-1; DJ1; HEL-S-67p; PARK7; SP22

查看更多收起

UniProt ID: (Human) Q99497, (Rat) O88767, (Mouse) Q99LX0

查看更多收起

Entrez Gene ID: (Human) 11315, (Rat) 117287, (Mouse) 57320

查看更多收起

core promoter binding double-stranded DNA binding single-stranded DNA binding transcription coactivator activity mRNA binding receptor binding copper ion binding protein binding transcription factor binding peptidase activity superoxide dismutase copper chaperone activity oxidoreductase activity, acting on peroxide as acceptor glyoxalase III activity enzyme binding kinase binding cytokine binding tyrosine 3-monooxygenase activator activity L-dopa decarboxylase activator activity identical protein binding protein homodimerization activity small protein activating enzyme binding ubiquitin-like protein conjugating enzyme binding mercury ion binding androgen receptor binding peroxiredoxin activity repressing transcription factor binding scaffold protein binding cadherin binding involved in cell-cell adhesion cupric ion binding cuprous ion binding ubiquitin-specific protease binding glyoxalase (glycolic acid-forming) activity molecular_function RNA binding histone deacetylase activity protein-N-terminal asparagine amidohydrolase activity UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity CoA hydrolase activity hydrolase activity iprodione amidohydrolase activity (3,5-dichlorophenylurea)acetate amidohydrolase activity 4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity didemethylisoproturon amidohydrolase activity N-isopropylacetanilide amidohydrolase activity N-cyclohexylformamide amidohydrolase activity 2-oxoglutaryl-CoA thioesterase activity 2,4,4-trimethyl-3-oxopentanoyl-CoA thioesterase activity isonicotinic acid hydrazide hydrolase activity cis-aconitamide amidase activity gamma-N-formylaminovinylacetate hydrolase activity 3-isopropylbut-3-enoyl-CoA thioesterase activity N2-acetyl-L-lysine deacetylase activity O-succinylbenzoate synthase activity indoleacetamide hydrolase activity N-acetylcitrulline deacetylase activity glutaryl-CoA hydrolase activity N-acetylgalactosamine-6-phosphate deacetylase activity acyl-CoA hydrolase activity diacetylchitobiose deacetylase activity chitooligosaccharide deacetylase activity

negative regulation of protein phosphorylation synaptic transmission, dopaminergic negative regulation of protein kinase activity proteolysis protein deglycosylation autophagy inflammatory response response to oxidative stress mitochondrion organization single fertilization adult locomotory behavior fertilization detoxification of copper ion negative regulation of gene expression methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione lactate biosynthetic process negative regulation of protein ubiquitination negative regulation of protein binding negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of interleukin-8 production positive regulation of peptidyl-serine phosphorylation negative regulation of protein sumoylation cellular response to oxidative stress cellular response to reactive oxygen species cellular response to glyoxal response to drug response to hydrogen peroxide hydrogen peroxide metabolic process negative regulation of apoptotic process regulation of neuron apoptotic process negative regulation of neuron apoptotic process regulation of TRAIL receptor biosynthetic process positive regulation of transcription from RNA polymerase II promoter glycolate biosynthetic process negative regulation of protein export from nucleus regulation of inflammatory response detoxification of mercury ion protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of ubiquitin-protein transferase activity dopamine uptake involved in synaptic transmission regulation of mitochondrial membrane potential membrane depolarization oxidation-reduction process membrane hyperpolarization negative regulation of cell death regulation of androgen receptor signaling pathway cellular response to hydrogen peroxide positive regulation of protein homodimerization activity positive regulation of protein localization to nucleus negative regulation of neuron death positive regulation of superoxide dismutase activity negative regulation of protein acetylation positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of mitochondrial electron transport, NADH to ubiquinone negative regulation of protein K48-linked deubiquitination negative regulation of TRAIL-activated apoptotic signaling pathway positive regulation of pyrroline-5-carboxylate reductase activity positive regulation of tyrosine 3-monooxygenase activity positive regulation of dopamine biosynthetic process glyoxal catabolic process positive regulation of L-dopa biosynthetic process positive regulation of L-dopa decarboxylase activity negative regulation of oxidative stress-induced cell death negative regulation of hydrogen peroxide-induced cell death negative regulation of hydrogen peroxide-induced neuron death negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway positive regulation of reactive oxygen species biosynthetic process negative regulation of ubiquitin-specific protease activity positive regulation of oxidative phosphorylation uncoupler activity positive regulation of transcription regulatory region DNA binding positive regulation of androgen receptor activity negative regulation of extrinsic apoptotic signaling pathway negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway cell proliferation positive regulation of gene expression enzyme active site formation via L-cysteine sulfinic acid negative regulation of protein catabolic process negative regulation of oxidative stress-induced neuron death Ras protein signal transduction protein repair activation of protein kinase B activity positive regulation of protein kinase B signaling cell-cell adhesion cellular oxidant detoxification regulation of fibril organization negative regulation of death-inducing signaling complex assembly positive regulation of mitophagy negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway