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Invitrogen
PARN Monoclonal Antibody (11D8)
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PARN Antibody (MA5-49199) in ICC/IF
Immunocytochemistry analysis of PARN in U251 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in PARN Monoclonal antibody (Product # MA5-49199) using a dilution of 2 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More
产品信息
MA5-49199
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
11D8
抗原
E. coli-derived human PARN recombinant protein (Position: M1-Y301).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human 293T whole cell, monkey COS-7 whole cell, human CACO-2 whole cell, rat kidney tissue, rat C6 whole cell, mouse NIH/3T3 whole cell. IHC: human mammary cancer tissue. ICC/IF: A549 cell, U251 cell. Flow: A549 cell, A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Deadenylating nuclease; Deadenylation nuclease; poly(A)-specific ribonuclease (deadenylation nuclease); Poly(A)-specific ribonuclease PARN; Polyadenylate-specific ribonuclease
基因别名: 1200003I18Rik; DAN; DKCB6; PARN; PFBMFT4; RGD1565449
UniProt ID: (Human) O95453, (Mouse) Q8VDG3
Entrez Gene ID: (Human) 5073, (Mouse) 74108, (Rat) 360464
