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Invitrogen
PARP1 Polyclonal Antibody
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PARP1 Antibody (PA5-34803) in ICC/IF
Immunofluorescence analysis of PARP was performed using log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PARP Polyclonal Antibody (Product # PA5-34803) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 f... View More
产品信息
PA5-34803
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.10 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: 293T, A431, HeLa, HepG2, HCT116, HCT116 (30 µM cisplatin treatment for 24 hr), NIH-3T3, PC-12, H1299.
Predicted reactivity: Mouse (90%), Rat (88%), Bovine (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; Protein poly-ADP-ribosyltransferase PARP1
基因别名: 5830444G22Rik; Adprp; ADPRT; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; pADPRT-1; PARP; PARP-1; PARP1; PPOL; sPARP-1
UniProt ID: (Human) P09874
Entrez Gene ID: (Human) 142, (Mouse) 11545
DNA binding
DNA ligase (ATP) activity
NAD+ ADP-ribosyltransferase activity
protein binding
transcription factor binding
zinc ion binding
enzyme binding
protein kinase binding
identical protein binding
histone deacetylase binding
poly(A) RNA binding
protein N-terminus binding
NAD binding
R-SMAD binding
chromatin binding
transferase activity
transferase activity, transferring glycosyl groups
metal ion binding
DNA metabolism protein
negative regulation of transcription from RNA polymerase II promoter
nucleotide-excision repair, DNA damage recognition
nucleotide-excision repair, DNA duplex unwinding
double-strand break repair via homologous recombination
lagging strand elongation
DNA repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
double-strand break repair
transcription from RNA polymerase II promoter
protein ADP-ribosylation
cellular response to DNA damage stimulus
mitochondrion organization
transforming growth factor beta receptor signaling pathway
positive regulation of cardiac muscle hypertrophy
protein autoprocessing
protein sumoylation
signal transduction involved in regulation of gene expression
macrophage differentiation
mitochondrial DNA metabolic process
cellular response to insulin stimulus
nucleotide-excision repair, DNA incision
cellular response to oxidative stress
protein modification process
DNA damage response, detection of DNA damage
mitochondrial DNA repair
positive regulation of transcription from RNA polymerase II promoter
DNA ligation involved in DNA repair
positive regulation of SMAD protein import into nucleus
protein poly-ADP-ribosylation
global genome nucleotide-excision repair
regulation of cellular protein localization
negative regulation of telomere maintenance via telomere lengthening
ATP generation from poly-ADP-D-ribose
positive regulation of transcription regulatory region DNA binding
telomere maintenance
DNA metabolic process
base-excision repair
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of growth rate
behavioral response to cocaine
cellular response to superoxide
regulation of single strand break repair
