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Invitrogen

PARP1 Recombinant Mouse Monoclonal Antibody (HC2R8)

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产品信息

740247M

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000
-

免疫细胞化学 (ICC/IF)

1:200
-

免疫沉淀 (IP)

20 µg
-
产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

HC2R8

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

规格

100 µg

纯化类型

Protein A/G

保存液

PBS, pH 7.2-7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

产品详细信息

Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase family, member 1; Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; Protein poly-ADP-ribosyltransferase PARP1; unnamed protein product

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基因别名: ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PARP1; PARS; Poly-PARP; PPOL

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UniProt ID: (Human) P09874

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Entrez Gene ID: (Human) 142

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功能
DNA binding chromatin binding damaged DNA binding RNA binding catalytic activity NAD+ ADP-ribosyltransferase activity protein binding enzyme activator activity zinc ion binding transferase activity transferase activity, transferring glycosyl groups nucleotidyltransferase activity enzyme binding protein kinase binding estrogen receptor binding nucleosome binding ubiquitin protein ligase binding identical protein binding protein homodimerization activity histone deacetylase binding metal ion binding NAD binding RNA polymerase II sequence-specific DNA binding transcription factor binding R-SMAD binding NAD DNA ADP-ribosyltransferase activity transcription regulator activator activity NAD+-protein-serine ADP-ribosyltransferase activity NAD+-protein-aspartate ADP-ribosyltransferase activity NAD+-protein-glutamate ADP-ribosyltransferase activity NAD+-protein-tyrosine ADP-ribosyltransferase activity NAD+-protein-histidine ADP-ribosyltransferase activity NAD+-histone H2BS6 serine ADP-ribosyltransferase activity NAD+-histone H3S10 serine ADP-ribosyltransferase activity NAD+-histone H2BE35 glutamate ADP-ribosyltransferase activity protein ADP-ribosylase activity
参与通路
negative regulation of transcription from RNA polymerase II promoter telomere maintenance double-strand break repair via homologous recombination immune system process DNA repair double-strand break repair transcription from RNA polymerase II promoter apoptotic process cellular response to DNA damage stimulus mitochondrion organization transforming growth factor beta receptor signaling pathway response to gamma radiation positive regulation of cardiac muscle hypertrophy carbohydrate biosynthetic process protein autoprocessing signal transduction involved in regulation of gene expression macrophage differentiation DNA ADP-ribosylation mitochondrial DNA metabolic process positive regulation of DNA-templated transcription, elongation cellular response to insulin stimulus regulation of protein localization positive regulation of intracellular estrogen receptor signaling pathway negative regulation of transcription elongation from RNA polymerase II promoter cellular response to oxidative stress cellular response to UV protein modification process positive regulation of I-kappaB kinase/NF-kappaB signaling mitochondrial DNA repair innate immune response regulation of circadian sleep/wake cycle, non-REM sleep negative regulation of innate immune response negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter decidualization positive regulation of mitochondrial depolarization positive regulation of SMAD protein import into nucleus positive regulation of necroptotic process protein poly-ADP-ribosylation protein auto-ADP-ribosylation protein localization to chromatin cellular response to zinc ion cellular response to transforming growth factor beta stimulus replication fork reversal DNA repair-dependent chromatin remodeling negative regulation of cGAS/STING signaling pathway transcription pausing by RNA polymerase II positive regulation of protein localization to nucleus cellular response to oxygen-containing compound regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway response to aldosterone negative regulation of adipose tissue development positive regulation of adipose tissue development negative regulation of telomere maintenance via telomere lengthening cellular response to beta-amyloid positive regulation of myofibroblast differentiation regulation of base-excision repair positive regulation of double-strand break repair via homologous recombination cellular response to nerve growth factor stimulus protein localization to site of double-strand break ATP generation from poly-ADP-D-ribose negative regulation of ATP biosynthetic process
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