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Invitrogen
PARP1 (cleaved p25) Recombinant Rabbit Monoclonal Antibody (9B10)
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PARP1 (cleaved p25) Antibody (MA572341) in IHC (P)
Immunohistochemistry analysis of PARP1 (cleaved p25) on paraffin-embedded Human Ovarian Cancer tissue. Antigen retrieval by boiling in sodium citrate buffer (pH 6.0) for 15 min. Samples were incubated with PARP1 (cleaved p25) Recombinant Monoclonal (Product # MA572341) using a dilution at 1:200 overnight at 4°C. Followed by DAB staining.
产品信息
MA572341
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
9B10
抗原
KLH conjugated synthetic peptide derived from human PARP1.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
TBS, pH 7.4, with 50% glycerol, 1% BSA
内含物
0.02% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
Poly (ADP ribose) polymerase 1 (PARP1) is a critical enzyme involved in DNA damage repair and the regulation of transcription, replication, and cell fate, becoming activated by DNA strand breaks to catalyze poly(ADP ribosyl)ation of itself and other DNA damage response proteins. During apoptosis, PARP1 is cleaved by caspase 3 into an 89 kDa N terminal fragment containing the DNA-binding zinc finger domains and a 24 to 25 kDa C terminal fragment known as cleaved PARP1 (p25), which primarily contains the catalytic domain; this cleavage separates the functional domains, inactivates PARP1s DNA repair capability, and represents a hallmark of programmed cell death. Biologically, PARP1 cleavage functions as a molecular switch from a pro-survival to a pro death state by preventing futile repair of severely damaged DNA, and clinically, increased levels of cleaved PARP1 (p25) are widely used as a marker of apoptosis in research and diagnostic settings, particularly in response to stimuli such as chemotherapy, radiotherapy, and other apoptotic signals.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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