Invitrogen

PAX6 Polyclonal Antibody

查看其他43个PAX6抗体

问题与答案

问题与答案

抗体检测数据 (3)

PAX6 Antibody in Western Blot (WB)

PAX6 Antibody in Western Blot (WB)

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PAX6 Antibody (PA5-18713) in WB

Western blot analysis of PAX6 using PAX6 Polyclonal Antibody (Product # PA5-18713) (0.3 µg/mL) in staining of U251 (A), (0.03 µg/mL) nuclear K562 (B) and (1 µg/mL) nuclear HeLa (C) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

PAX6 Antibody in Western Blot (WB)

PAX6 Antibody in Western Blot (WB)

PAX6 Antibody in Flow Cytometry (Flow)

Product Image Gallery

PAX6 Antibody in Western Blot (WB) — Western blot analysis of PAX6 using PAX6 Polyclonal Antibody (Product # PA5-18713) (0.3 µg/mL) in staining of U251 (A), (0.03 µg/mL) nuclear K562 (B) and (1 µg/mL) nuclear HeLa (C) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

PAX6 Antibody in Western Blot (WB) — Western blot analysis of PAX6 using PAX6 Polyclonal Antibody (Product # PA5-18713) (0.5 µg/mL) in staining of RAW264.7 cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

PAX6 Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of PAX6 using PAX6 Polyclonal Antibody (Product # PA5-18713) in paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunogen image

PAX6 Polyclonal Antibody

Immunogen Image PAX6 Polyclonal Antibody

产品信息

PA5-18713

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.03-1 µg/mL

-

流式细胞分析 (Flow)

10 µg/mL

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (REEKLRNQRRQASN) corresponding to the internal amino acids of PAX6 (aa 267-281).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

This antibody is predicted to react with human and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

靶标信息

This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Aniridia; Aniridia type II protein; Dickie's small eye; Keratitis; MGC17209; Oculorhombin; paired box gene 6; Paired Box Gene-6; paired box homeotic gene-6; Paired box protein Pax-6; small eye

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基因别名: 1500038E17Rik; AEY11; AN; AN2; D11S812E; Dey; FVH1; Gsfaey11; MGDA; Pax-6; PAX6; Sey; WAGR

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UniProt ID: (Human) P26367, (Mouse) P63015

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Entrez Gene ID: (Human) 5080, (Mouse) 18508

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RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding ubiquitin-protein transferase activity protein binding transcription factor binding protein kinase binding ubiquitin protein ligase binding histone acetyltransferase binding co-SMAD binding R-SMAD binding HMG box domain binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding AT DNA binding double-stranded DNA binding sequence-specific DNA binding transcription regulatory region DNA binding

negative regulation of transcription from RNA polymerase II promoter establishment of mitotic spindle orientation blood vessel development eye development cell fate determination neuron migration negative regulation of protein phosphorylation positive regulation of neuroblast proliferation lens development in camera-type eye type B pancreatic cell differentiation pancreatic A cell development transcription from RNA polymerase II promoter smoothened signaling pathway axon guidance central nervous system development salivary gland morphogenesis visual perception response to wounding regulation of asymmetric cell division organ morphogenesis dorsal/ventral axis specification positive regulation of gene expression protein ubiquitination oligodendrocyte cell fate specification cerebral cortex regionalization forebrain anterior/posterior pattern specification forebrain dorsal/ventral pattern formation commitment of neuronal cell to specific neuron type in forebrain forebrain-midbrain boundary formation regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification regulation of transcription from RNA polymerase II promoter involved in somatic motor neuron fate commitment pituitary gland development signal transduction involved in regulation of gene expression keratinocyte differentiation regulation of cell migration positive regulation of epithelial cell differentiation lacrimal gland development protein localization to organelle eye photoreceptor cell development glucose homeostasis negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of timing of cell differentiation embryonic camera-type eye morphogenesis neuron fate commitment astrocyte differentiation negative regulation of epithelial cell proliferation negative regulation of neurogenesis retina development in camera-type eye iris morphogenesis cornea development in camera-type eye negative regulation of neural precursor cell proliferation neural crest cell migration epithelial cell development pancreatic A cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter multicellular organism development axonogenesis brain development negative regulation of cell proliferation anterior/posterior pattern specification dorsal/ventral pattern formation regulation of gene expression regulation of neuron projection development pallium development rhombomere morphogenesis olfactory bulb development telencephalon regionalization interkinetic nuclear migration cell differentiation forebrain development hindbrain development positive regulation of cell fate specification camera-type eye development cell fate commitment regulation of neuron differentiation regulation of neurogenesis neural precursor cell proliferation positive regulation of glucagon secretion insulin metabolic process positive regulation of neuron migration