Invitrogen

PAX8 Monoclonal Antibody (1F8-3A8)

Name: PAX8 Monoclonal Antibody (1F8-3A8)
Brand: Invitrogen
SKU: MA1-117

高级验证

3个已发表图片

7 篇参考文献

查看其他65个PAX8抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

PAX8 Antibody in Immunocytochemistry (ICC/IF)

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PAX8 Antibody (MA1-117) in ICC/IF

Immunofluorescent analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a PAX8 monoclonal antibody (Product # MA1-117) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) a... View More

PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PAX8 Antibody in Immunohistochemistry (IHC)

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PAX8 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. COS-7 cells were transfected with PAX8 siRNA and reduction of signal was observed in Western Blot using PAX8 Monoclonal Antibody (Product # MA1-117). {KD}

产品信息

MA1-117

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:200

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

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产品规格

种属反应

Human, Mouse, Non-human primate

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1F8-3A8

抗原

Purified internal fragment of human recombinant PAX8 expressed in E. coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536828

产品详细信息

Western blot analysis of MA1-117 detects a prominent ~48 kDa protein in human adenocarcinoma SKOV-3 and SV40 transformed African green monkey (Cercopithecus aethiops) kidney cells. In SKOV-3 cells, additional unknown low molecular bands are also detected. Specificity of the antibody was confirmed in HeLa (negative control) and 293T cells overexpressing full length PAX8.

靶标信息

This gene encodes a member of the paired box family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.

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