Bethyl Laboratories

PBRM1 Recombinant Rabbit Monoclonal Antibody (BL-39-2C3)

Name: PBRM1 Recombinant Rabbit Monoclonal Antibody (BL-39-2C3)
Brand: Bethyl Laboratories
SKU: A700-019

查看其他15个PBRM1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (8)

PBRM1 Antibody in Immunocytochemistry (ICC/IF)

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PBRM1 Antibody (A700-019) in ICC/IF

Detection of human PBRM1 in FFPE MCF7 cells by immunocytochemistry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019) (lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

PBRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PBRM1 Antibody in Immunoprecipitation (IP)

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Immunogen image

Immunogen Image PBRM1 Recombinant Rabbit Monoclonal Antibody (BL-39-2C3)

产品信息

A700-019

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫细胞化学 (ICC/IF)

1:100-1:500

流式细胞分析 (Flow)

0.8 µL per 1 x 10^6 cells

免疫沉淀 (IP)

5 µL/mg Lysate

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

BL-39-2C3

抗原

Recombinant protein representing residues 1-293 of human Polybromo 1.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

purified

保存液

BBS, pH 8.2, with 0.1% BSA

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For ICC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

靶标信息

The PBRM1 gene encodes a subunit of the PBAF (Polybromo-associated BAF) chromatin remodeling complex, playing a crucial role in the regulation of chromatin structure and transcription. PBRM1, also known as BAF180, contains bromodomains that recognize acetylated lysine residues on histone tails, facilitating chromatin remodeling necessary for effective transcriptional regulation. This gene is frequently mutated in renal cell carcinoma, particularly clear cell renal cell carcinoma (ccRCC), where such mutations are implicated in the pathogenesis of the disease. PBRM1 acts as a tumor suppressor, and its loss of function can lead to deregulation of gene expression, contributing to oncogenic processes. It is involved in pathways that govern cell cycle regulation and DNA damage response, reflecting its importance in maintaining genomic stability. Ongoing research focuses on understanding PBRM1's interactions within chromatin remodeling complexes and its potential as a therapeutic target in cancer treatment.

仅用于科研。不用于诊断过程。未经明确授权不得转售。