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NeoBiotechnologies
PCNA (Proliferating Cell Nuclear Antigen) Monoclonal Antibody (PCNA, 694)
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产品信息
5111-MSM2-P1
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
PCNA, 694
抗原
Recombinant full length human PCNA protein
偶联物
形式
Liquid
浓度
200 µg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.4, with 0.05% BSA
内含物
0.05% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Immunohistochemistry (PFA fixed): incubate antibody for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
This antibody shows a broad species reactivity.
靶标信息
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen
基因别名: ATLD2; PCNA
UniProt ID: (Human) P12004
Entrez Gene ID: (Dog) 477166, (Human) 5111
purine-specific mismatch base pair DNA N-glycosylase activity
chromatin binding
damaged DNA binding
protein binding
enzyme binding
estrogen receptor binding
DNA polymerase processivity factor activity
receptor tyrosine kinase binding
dinucleotide insertion or deletion binding
MutLalpha complex binding
histone acetyltransferase binding
identical protein binding
DNA polymerase binding
DNA polymerase processivity factor
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
telomere maintenance via recombination
telomere maintenance
DNA strand elongation involved in DNA replication
leading strand elongation
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
mismatch repair
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
heart development
cell proliferation
protein sumoylation
translesion synthesis
epithelial cell differentiation
replication fork processing
positive regulation of deoxyribonuclease activity
nucleotide-excision repair, DNA incision
response to lipid
cellular response to UV
error-prone translesion synthesis
DNA damage response, detection of DNA damage
positive regulation of DNA repair
positive regulation of DNA replication
response to cadmium ion
error-free translesion synthesis
mitotic telomere maintenance via semi-conservative replication
