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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PDGF-B Antibodies

          Invitrogen

          PDGF-B Polyclonal Antibody

          1 Reference
          View all (15) PDGF-B antibodies

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          Cite PDGF-B Polyclonal Antibody

          • Antibody Testing Data (2)
          PDGF-B Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          PDGF-B Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          PDGF-B Antibody (PA5-19524) in WB

          Western Blot was performed using Anti-PDGF-B Polyclonal Antibody (Product # PA5-19524) and a 17 kDa band corresponding to Platelet-derived growth factor subunit B was observed across cell lines tested and was upregulated in cell lines upon PTI treatment. Also, the expression was found to be high in HUVEC when compared to SH-SH5Y. Whole cell extracts (30 µg lysate) of HMEC-1 (Lane 1), HUVEC (Lane 2), HUVEC treated with PTI (1X for 4 hrs) (Lane 3), SH-SY5Y (Lane 4), SH-SY5Y treated with PTI (1X for 4 hrs) (Lane 5) were electrophoresed using ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PDGF-B Antibody in Western Blot (WB)
          PDGF-B Antibody in Western Blot (WB)
          PDGF-B Polyclonal Antibody

          Product Details

          PA5-19524

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          5 µg/mL
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:150
          -

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PDGF B.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.8 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 1% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10980265

          Product Specific Information

          This antibody is predicted to react with rat and cow based on sequence homology.

          Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.

          Target Information

          This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: becaplermin; c-sis; PDGF subunit B; PDGF, B chain; PDGF-2; platelet-derived growth factor 2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); Platelet-derived growth factor subunit B; platelet-derived growth factor, beta polypeptide (oncogene SIS); Proto-oncogene c-Sis

          View more View less

          Gene Aliases: c-sis; IBGC5; PDGF-2; PDGF-B; PDGF2; PDGFB; SIS; SSV

          View more View less

          UniProt ID: (Human) P01127, (Mouse) P31240

          View more View less

          Entrez Gene ID: (Human) 5155, (Mouse) 18591

          View more View less

          Function(s)
          Ras guanyl-nucleotide exchange factor activity platelet-derived growth factor receptor binding protein binding collagen binding growth factor activity superoxide-generating NADPH oxidase activator activity chemoattractant activity identical protein binding protein homodimerization activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity platelet-derived growth factor binding receptor binding growth factor
          Process(es)
          MAPK cascade response to hypoxia embryonic placenta development positive regulation of endothelial cell proliferation monocyte chemotaxis platelet degranulation positive regulation of glomerular filtration DNA replication protein phosphorylation transforming growth factor beta receptor signaling pathway heart development positive regulation of cell proliferation response to wounding negative regulation of phosphatidylinositol biosynthetic process negative regulation of platelet activation positive regulation of gene expression negative regulation of gene expression regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of smooth muscle cell migration cell growth peptidyl-serine phosphorylation peptidyl-tyrosine phosphorylation hemopoiesis extracellular matrix organization positive regulation of cell migration positive regulation of protein autophosphorylation negative regulation of protein binding activation of protein kinase activity activation of protein kinase B activity response to estradiol response to insulin positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway paracrine signaling wound healing response to drug positive regulation of MAP kinase activity positive regulation of MAPK cascade positive regulation of blood vessel endothelial cell migration positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity response to estrogen positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of DNA replication positive regulation of mitotic nuclear division negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated phosphatidylinositol phosphorylation platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation positive regulation of smooth muscle cell proliferation positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of chemotaxis positive regulation of cell division cell chemotaxis positive regulation of protein tyrosine kinase activity positive regulation of ERK1 and ERK2 cascade protein kinase C signaling cellular response to growth factor stimulus cellular response to mycophenolic acid positive regulation of glomerular mesangial cell proliferation metanephric glomerular mesangial cell development reactive oxygen species metabolic process positive regulation of calcium ion import positive regulation of hyaluronan biosynthetic process negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of vascular smooth muscle cell proliferation positive regulation of vascular associated smooth muscle cell migration negative regulation of vascular smooth muscle cell differentiation positive regulation of vascular smooth muscle cell dedifferentiation positive regulation of reactive oxygen species metabolic process positive regulation of DNA biosynthetic process positive regulation of metanephric mesenchymal cell migration substrate-dependent cell migration multicellular organism development synapse assembly neuron remodeling glial cell development cell projection assembly actin cytoskeleton organization negative regulation of cell migration positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway eye photoreceptor cell development positive regulation of fibroblast growth factor receptor signaling pathway positive regulation of vasoconstriction negative regulation of vasodilation positive regulation of mitotic cell cycle, embryonic blood vessel morphogenesis regulation of peptidyl-tyrosine phosphorylation retina development in camera-type eye branching involved in salivary gland morphogenesis epithelial cell proliferation involved in salivary gland morphogenesis cardiac vascular smooth muscle cell differentiation retina vasculature development in camera-type eye metanephric glomerular mesangial cell proliferation involved in metanephros development
          It has to be done as per old AB suggested Products section.
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