Invitrogen

PDH Antibody Cocktail

Name: PDH Antibody Cocktail
Brand: Invitrogen
SKU: 45-6799

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)

PDH Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

PDH Antibody (45-6799) in ICC/IF

Immunofluorescence analysis of PDH was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with PDH Antibody Cocktail (Product # 45-6799) at 1:250 dilution in 0.1% BSA, incubated at 4°C overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A11029, 1:2,000 dilution) for 4... View More

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产品信息

45-6799

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫细胞化学 (ICC/IF)

1:250

产品规格

种属反应

Bovine, Human, Mouse, Rat

宿主/亚型

Mouse / Ig

分类

Cocktail

类型

Antibody

克隆号

Cocktail

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.5 mg/mL

纯化类型

IgG fraction

保存液

HEPES buffered saline, pH 7.5

内含物

0.02% sodium azide

保存条件

4°C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_2533826

产品详细信息

Anti-PDH Western Blot Kit; Western Blot kit (Cocktail of monoclonal antibodies) specific to Human, bovine PDH. This antibody is validated for use in Western Blot.

C-V-OSCP (clone ) 4C11C10D12 - Antigen Human rec. mature OSCP
Amount: 12.5 µg , Working concentration: 0.5 µg/mL

PDH-E1-alpha (clone) 9H9AF5 - Human rec. PDH-E1-a
Amount: 25 µg , Working concentration: 1 µg/mL

PDH-E1-beta (clone) 17A5E2H8 - Porcine PDH
Amount: 62.5 µg, Working concentration: 2.5 µg/mL

PDH-E2 (clone) 15D3G9C11 - Porcine PDH
Amount: 25 µg, Working concentration: 1 µg/mL

PDH-E2/E3bp (clone) 13G2AE2BH5 - Porcine PDH
Amount: 25 µg, Working concentration: 1 µg/mL

靶标信息

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 13G2AE2BH5; 15D3G9C11; 17A5E2H8; 4C11C10D12; 9H9AF5; PDHE1-A type I; PDHE1-A type II; PDHE1-B; pyruvate dehydrogenase; pyruvate dehydrogenase (lipoamide) alpha 1; pyruvate dehydrogenase (lipoamide) alpha 2; pyruvate dehydrogenase (lipoamide) beta; pyruvate dehydrogenase alpha 1; pyruvate dehydrogenase alpha 2; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; pyruvate dehydrogenase E1 alpha 1; pyruvate dehydrogenase E1 alpha 1 subunit; pyruvate dehydrogenase E1 alpha 2; pyruvate dehydrogenase E1 alpha 2 subunit; pyruvate dehydrogenase E1 beta subunit; pyruvate dehydrogenase E1 component alpha subunit; PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, TESTIS-SPECIFIC FORM, MITOCHONDRIAL PRECURSOR (PDHE1-A TYPE II); Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial; Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; pyruvate dehydrogenase E1 subunit; pyruvate dehydrogenase E1alpha subunit; pyruvate dehydrogenase, E1 beta polypeptide; pyruvate dehydrogenase, E1-alpha polypeptide, testis specific; RP11-723P2.1

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基因别名: 2610103L06Rik; AL024199; C81408; PDHA; Pdha-1; Pdha-2; PDHA1; PDHA2; PDHAD; PDHAL; PDHB; PDHBD; PDHCE1A; PDHE1-B; PHE1A; PHE1B