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Agrisera
PDHE1-A/Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial Polyclonal Antibody
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PDHE1-A/Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial Antibody (AS23-4974) in WB
Samples: 1 - 40 µg of Arabidopsis thaliana Col-0 whole leaf extract. 2 - 40 µg of Arabidopsis thaliana mutant iar 4 (AT 1 G 24180), Arabidopsis thaliana pyruvate dehydrogenase E 1 a-like subunit. 3 - 40 µg of Arabidopsis thaliana mutant iar 4 l-1 (AT 1 G 59900), Arabidopsis thaliana pyruvate dehydrogenase E 1 a subunit. 4 - 40 µg of Arabidopsis thaliana mutant iar 4 l-2 (AT 1 G 59900), Arabidopsis thaliana ... View More
产品信息
AS23-4974
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated, unique peptide derived from Arabidopsis thaliana PDHE1-A.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
PDHE1-A (PDHA1) encodes the E1 component alpha subunit of the nuclear-encoded pyruvate dehydrogenase (PDH) complex and is synthesized as a mitochondrial precursor that is imported into the mitochondrial matrix after cleavage of an N-terminal targeting peptide. The mature protein assembles with the E1 beta subunit to form an alpha2beta2 heterotetrameric E1 enzyme; this thiamine diphosphate (ThDP)- and Mg2+-dependent decarboxylase provides the first catalytic step of the PDH complex, binding/coordinating ThDP and forming the active-site architecture required for pyruvate oxidative decarboxylation. Functionally, E1 (via the PDHE1-A-containing heterotetramer) catalyzes ThDP-mediated decarboxylation of pyruvate and generation of a hydroxyethyl-ThDP intermediate, enabling subsequent transfer of the acetyl group to the lipoyl moiety on the E2 component and ultimately production of acetyl-CoA and NADH; in this way it constitutes a key biochemical link between cytosolic glycolysis and the tricarboxylic acid cycle/oxidative phosphorylation. PDHE1-A activity is tightly regulated by reversible phosphorylation on serine residues located in flexible regulatory loops of the alpha subunit, where phosphorylation promotes loop disorder and/or restricts substrate-channel access, thereby inhibiting E1 catalysis and limiting acetyl transfer to the E2 lipoyl domain.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E1alpha; PDHA; PDHAD; PDHCE1A; PHE1A
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