Invitrogen

PDK1 Monoclonal Antibody (4A11)

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产品信息

MA5-15797

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:2,000
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免疫组化 (IHC)

-
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免疫组化（石蜡） (IHC (P))

1:200-1:1,000
-

免疫细胞化学 (ICC/IF)

1:200-1:1,000
-

流式细胞分析 (Flow)

1:200-1:400
-

酶联免疫吸附实验 (ELISA)

1:10,000
-

亲和纯化 (AP)

-
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产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

C. elegans, Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

4A11

抗原

Purified recombinant fragment of human PDK1 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11152628

产品详细信息

MA5-15797 targets PDK1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, Non-human primate, and Rat samples.

The MA5-15797 immunogen is purified recombinant fragment of human PDK1 expressed in E. Coli.

MA5-15797 detects PDK1 which has a predicted molecular weight of approximately 44kDa.

靶标信息

PDK1 (3-phosphoinositide dependent kinase-1) is a serine/threonine protein kinase localized to the plasma membrane. It plays a vital role in insulin-induced glycogen synthesis, PKC activation, and activation of the AGC subfamily of protein kinases. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; PDH kinase 1; PDK p48; PkB kinase like gene 1; pyruvate dehydrogenase; pyruvate dehydrogenase kinase 1; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1

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基因别名: B830012B01; D530020C15Rik; PDK1; PDPK1; PDPK2; PDPK2P; PRO0461

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UniProt ID: (Human) O15530, (Mouse) Q8BFP9

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Entrez Gene ID: (Human) 5170, (Rat) 116551, (Mouse) 228026

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功能
protein serine/threonine kinase activity 3-phosphoinositide-dependent protein kinase activity insulin receptor binding protein binding ATP binding phospholipase activator activity kinase activity protein kinase binding phospholipase binding protein kinase activity pyruvate dehydrogenase (acetyl-transferring) kinase activity protein complex binding protein homodimerization activity protein heterodimerization activity nucleotide binding transferase activity non-receptor serine/threonine protein kinase
参与通路
stimulatory C-type lectin receptor signaling pathway type B pancreatic cell development transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation negative regulation of protein kinase activity hyperosmotic response epidermal growth factor receptor signaling pathway positive regulation of phospholipase activity regulation of endothelial cell migration negative regulation of cardiac muscle cell apoptotic process cell migration peptidyl-serine phosphorylation peptidyl-threonine phosphorylation calcium-mediated signaling actin cytoskeleton organization platelet activation negative regulation of transforming growth factor beta receptor signaling pathway T cell costimulation activation of protein kinase B activity cellular response to insulin stimulus negative regulation of toll-like receptor signaling pathway intracellular signal transduction Fc-epsilon receptor signaling pathway regulation of I-kappaB kinase/NF-kappaB signaling regulation of mast cell degranulation protein autophosphorylation phosphatidylinositol-mediated signaling focal adhesion assembly T cell receptor signaling pathway positive regulation of release of sequestered calcium ion into cytosol cellular response to epidermal growth factor stimulus positive regulation of establishment of protein localization to plasma membrane extrinsic apoptotic signaling pathway glucose metabolic process cell surface receptor signaling pathway cell proliferation intrinsic apoptotic signaling pathway in response to oxidative stress regulation of acetyl-CoA biosynthetic process from pyruvate regulation of glucose metabolic process hypoxia-inducible factor-1alpha signaling pathway carbohydrate metabolic process phosphorylation mitophagy in response to mitochondrial depolarization
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