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Invitrogen
PDK3 Polyclonal Antibody
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PDK3 Antibody (PA5-120166) in WB
Western blot analysis of PDK3 in extracts of SH-SY5Y cells. Samples were incubated with PDK3 Polyclonal antibody (Product # PA5-120166) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-120166
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 137-406 of human PDK3 (NP_005382.1).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.6 mg/mL
规格
60 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive test controls include: SH-SY5Y. The target is usually found in the following locations: Mitochondrion matrix.
Immunogen sequence: FGFDPFISTN IQYFLDRFYT NRISFRMLIN QHTLLFGGDT NPVHPKHIGS IDPTCNVADV VKDAYETAKM LCEQYYLVAP ELEVEEFNAK APDKPIQVVY VPSHLFHMLF ELFKNSMRAT VELYEDRKEG YPAVKTLVTL GKEDLSIKIS DLGGGVPLRK IDRLFNYMYS TAPRPSLEPT RAAPLAGFGY GLPISRLYAR YFQGDLKLYS MEGVGTDAVI YLKALSSESF ERLPVFNKSA WRHYKTTPEA DDWSNPSSEP RDASKYKAKQ
靶标信息
PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial; pyruvate dehydrogenase; Pyruvate dehydrogenase kinase isoform 3
基因别名: PDHK3; PDK3
Entrez Gene ID: (Human) 5165
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