Invitrogen

PEDF Polyclonal Antibody

图: 1 / 2

PEDF Antibody (PA5-120483) in WB

Western blot analysis of PEDF in extracts of various cell lines. Samples were incubated with PEDF Polyclonal antibody (Product # PA5-120483) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Product Image Gallery

产品信息

PA5-120483

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence within amino acids 141-240 of human PEDF/SERPINF1 (NP_0026063)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.89 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2914055

产品详细信息

Positive test controls include: HepG2, Mouse liver, Human serum. The target is usually found in the following locations: Melanosome, Secreted.

Immunogen sequence: RIVFEKKLRI KSSFVAPLEK SYGTRPRVLT GNPRLDLQEI NNWVQAQMKG KLARSTKEIP DEISILLLGV AHFKGQWVTK FDSRKTSLED FYLDEERTVR

靶标信息

PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is a 50 kDa glycoprotein produced and secreted in many tissues throughout the body. A major component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. In addition, PEDF is able to inhibit the activity of angiogenic factors, such as VEGF and FGF-2. The neuroprotective effects of PEDF are achieved through suppression of neuronal apoptosis induced by peroxide, glutamate, or other neurotoxins. The recent identification of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity (1) should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. To date, PEDF-R is the only signaling receptor known to be used by a serpin family member. The unique range of PEDF activities implicate it as a potential therapeutic agent for the treatment of vasculature-related neurodegenerative diseases, such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF also has the potential to be useful in the treatment of various angiogenesis-related diseases including a number of cancers.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: alpha-2 antiplasmin; Caspin; Cell proliferation-inducing gene 35 protein; EPC 1; EPC-1; EPC1; PEDF; PIG 35; pigment epithelium derived factor; Pigment epithelium-derived factor; SDF-3; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; serine (or cysteine) proteinase inhibitor, clade F), member 1; serine (or cysteine) proteinase inhibitor, clade F, member 1; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; serpin-F1; SERPINF 1; sserpin family F member 1; stromal cell derived factor 3; Stromal cell-derived factor 3; testis tissue sperm-binding protein Li 70n

查看更多收起

基因别名: AI195227; EPC-1; OI12; OI6; PEDF; Pedfl; PIG35; Sdf3; SERPINF1

查看更多收起

UniProt ID: (Human) P36955, (Mouse) P97298

查看更多收起

Entrez Gene ID: (Human) 5176, (Mouse) 20317

查看更多收起

功能

serine-type endopeptidase inhibitor activity protein binding protease inhibitor

参与通路