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Invitrogen
PER1 Polyclonal Antibody
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FIGURE: 1 / 4
PER1 Antibody (PA5-119798) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of PER1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with PER1 Polyclonal Antibody (Product # PA5-119798) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-119798
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within human PER1 aa 291-340.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2 mg/mL
Amount
200 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.1% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: 293T cell lysate, Hela cell lysate, Daudi cell lysate, mouse pancreas tissue lysate, rat brain tissue lysate, Hela, rat kidney tissue.
Subcellular Location: Nucleus, Cytoplasm.
Target Information
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Circadian clock protein PERIOD 1; Circadian pacemaker protein Rigui; hPER1; mPER1; Period circadian protein homolog 1; period homolog 1
Gene Aliases: KIAA0482; m-rigui; mPer1; PER; PER1; RIGUI
UniProt ID: (Mouse) O35973
Entrez Gene ID: (Mouse) 18626
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, transcription factor binding
signal transducer activity
protein binding
transcription factor binding
kinase binding
chromatin DNA binding
ubiquitin protein ligase binding
E-box binding
transcription cofactor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
regulation of sodium ion transport
transcription, DNA-templated
regulation of transcription, DNA-templated
signal transduction
circadian rhythm
posttranscriptional regulation of gene expression
circadian regulation of gene expression
regulation of hair cycle
regulation of circadian rhythm
negative regulation of I-kappaB kinase/NF-kappaB signaling
entrainment of circadian clock by photoperiod
histone H3 acetylation
histone H4 acetylation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JNK cascade
rhythmic process
response to cAMP
histone H3 deacetylation
circadian regulation of translation
regulation of cytokine production involved in inflammatory response
regulation of p38MAPK cascade
negative regulation of glucocorticoid receptor signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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