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          • Primary Antibodies ›
          • PFK1-7 Antibodies

          Agrisera

          PFK1-7/Phosphofructokinase 1-7 Polyclonal Antibody

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          Cite PFK1-7/Phosphofructokinase 1-7 Polyclonal Antibody

          Additional Information:
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          • 抗体检测数据 (1)
          PFK1-7/Phosphofructokinase 1-7 Antibody in Western Blot (WB)
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          PFK1-7/Phosphofructokinase 1-7 Antibody in Western Blot (WB)
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          PFK1-7/Phosphofructokinase 1-7 Antibody (AS23-4914) in WB

          Samples: 20 µg of Arabidopsis thaliana whole leaf extract 20 µg of Arabidopsis thaliana mutant described above the picture MW markers are marked on the left side of each membrane 20 µg/well of total protein extracted freshly from leaf extracts of Arabidopsis thaliana wildtype and mutants. Exact buffer components were: 50 m M Hepes-KOH (p H 6.8), 5 m M Mg-acetate, 15% Glycerin, 1 m M EDTA, 1 m M EGTA, 5 m M ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          PFK1-7/Phosphofructokinase 1-7 Antibody in Western Blot (WB)

          产品信息

          AS23-4914

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000-1:2,000
          -
          产品规格

          种属反应

          Plant

          宿主/亚型

          Rabbit

          分类

          Polyclonal

          类型

          Antibody

          抗原

          KLH-conjugated peptide derived from Arabidopsis thaliana PFK1-7 protein sequences.

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Lyophilized

          规格

          50 µg

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS, pH 7.4

          内含物

          no preservative

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          产品详细信息

          Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

          For reconstitution add 50 µL of sterile water.

          Specific Species Reactivity: Arabidopsis thaliana

          靶标信息

          In Arabidopsis thaliana, the cytosolic ATP-dependent phosphofructokinase gene set PFK1-7 encodes soluble enzymes that localize predominantly to the cytosol, supporting leaf and whole-plant carbohydrate metabolism. These proteins are ~444-537 amino acids in length and share the conserved phosphofructokinase catalytic architecture required for ATP-dependent phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate, the major committed and highly regulated step of glycolysis. Genetic analyses indicate that multiple cytosolic isoforms (including PFK1, PFK3, PFK6, and PFK7) act redundantly yet are collectively important for maintaining sugar homeostasis and normal growth, consistent with a central role in balancing carbon flux between glycolysis and downstream biosynthetic and energy-producing pathways.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

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          引用该产品

          生物信息学

          蛋白别名: AT5G56630; AtPFK7; MIK19.8; MIK19_8; PFK7

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          It has to be done as per old AB suggested Products section.

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