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Agrisera
PGR5/Proton gradient regulation 5 Polyclonal Antibody
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PGR5/Proton gradient regulation 5 Antibody (AS16-3985) in WB
Thylakoids were isolated according to Sirpiö et al (2011, Methods Mol Biol. 2011; 775:19-30). 3 µg/µL of total chlorophyll from Arabidopsis thaliana col-0 (1) and Arabidopsis thaliana pgr 5 mutant (Munekage et al. 2002, Cell) (2) were denatured in LaemmLi buffer at room temperature (15 min) were separated on 12% SDS-PAGE mini-gels with 6 M urea and blotted for 1 h to a PVDF membrane using a semi-dry transfe... View More
产品信息
AS16-3985
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana PGR5.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Picea abies, Pinus sylvestris, Setaria viridis, Solanum lycopersicum
靶标信息
PGR5 encodes a small chloroplast thylakoid-membrane protein that localizes to thylakoids where photosystem I electron transport occurs and is genetically and physically linked to the antimycin A-sensitive cyclic electron flow machinery via association with the PGRL1 complex. In Arabidopsis, loss of PGR5 compromises formation of the trans-thylakoid proton gradient (Delta pH) and proton motive force during illumination, indicating that the protein is required for efficient cyclic electron flow around photosystem I and/or its regulation, with consequent effects on ATP supply. Functionally, PGR5 is essential for photoprotection: by supporting Delta pH-dependent energy dissipation (qE component of non-photochemical quenching) and photosynthetic control at the cytochrome b6f complex, it helps prevent over-reduction on the acceptor side of photosystem I and limits photosystem I photoinhibition, particularly under high or fluctuating light.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AtPGR5; proton gradient regulation 5; T20G20.3; T20G20_3
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