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Agrisera
PGRL1/PGR5-like protein 1A (chloroplastic) Polyclonal Antibody
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PGRL1/PGR5-like protein 1A (chloroplastic) Antibody (AS19-4311) in WB
Whole leaf protein (corresponding to 3 mg fresh weight) from the Arabidopsis thaliana lines Columbia-0 (Col-0), PGR 5 knock out (pgr 5-1), line-2 (line-2) and PGRL 1 A+B knock out (pgrl 1 ab) was extracted and denatured with 2 x Tricine sample buffer (100 m M Tris/HCl, 24% (w/v) glycerol, 8% SDS and 15 m M DTT) by heating for 5 min at 70°C. The samples were separated on a 10% Tris Tricine gel and blotted ov... View More
产品信息
AS19-4311
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana PGRL1A.Peptide used to elicit this antibody is conserved in both isoforms PGRL1A and 1B of Zea mays.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Picea abies, Pinus sylvestris
靶标信息
PGRL1A encodes a chloroplast thylakoid-membrane protein that localizes to the photosynthetic electron-transfer membranes, where it physically associates with photosystem I and forms a functional complex with PGR5 to support cyclic electron flow around photosystem I. Structurally, PGRL1A is an integral membrane protein with transmembrane segment(s) anchoring it in the thylakoid and a cysteine-rich region important for redox chemistry; biochemical work indicates that conserved redox-active cysteines and an iron-containing cofactor are required for its activity and regulation. Functionally, the PGR5-PGRL1A pathway mediates antimycin A-sensitive cyclic electron flow by channeling electrons from ferredoxin back to the plastoquinone pool (i.e., ferredoxin:plastoquinone reductase activity attributed to PGRL1), thereby enhancing proton motive force generation without net NADPH production, helping tune the ATP/NADPH balance and contributing to photoprotection under fluctuating or high light by supporting lumen acidification-dependent control of electron transport.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT4G22890; F7H19.70; F7H19_70; PGR5-LIKE A; PGRL1A
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