Invitrogen

PHF14 Polyclonal Antibody

1 篇参考文献

查看其他4个PHF14抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (2)
高级验证 (1)

PHF14 Antibody in Immunocytochemistry (ICC/IF)

PHF14 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

PHF14 Antibody (PA5-72775) in ICC/IF

Immunofluorescence of PHF14 in 293 cells with PHF14 Polyclonal Antibody (Product # PA5-72775) at 5 µg/mL.

PHF14 Antibody in Immunocytochemistry (ICC/IF)

PHF14 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

PHF14 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence of PHF14 in 293 cells with PHF14 Polyclonal Antibody (Product # PA5-72775) at 5 µg/mL.

PHF14 Antibody in Western Blot (WB) — Western Blot analysis of PHF14 in 293 cell lysate with PHF14 Polyclonal Antibody (Product # PA5-72775) at 1 µg/mL.

PHF14 Antibody — PHF14 silencing in glioma cell lines decelerates proliferation and clonogenic growth. a, b The knockdown efficacy of PHF14 examined in U251, U87MG and A172 cells by qRT-PCR (a) and Western blot (b). c, d Cellular DNA replication is mitigated after PHF14 knocked down. Ten thousand U87MG, U251 and A172 cells per well were seeded in 96-well plates overnight. EdU and Hoechest co-staining was performed on the second day to assess cellular DNA replication activities (c) (Scale bar=100 µm). Values are counted as EdU+ cells/total cells (d). e-g PHF14 silenced cells showed weaker proliferation and colony formation potential. 500 U251 and A172 cells per well were cultured in 6-well plates in Complete growth medium for 10 days and then stained by 1% crystal violet for colony formation assay (e) and single colony morphology (f) (Scale bar=200 µm). The number of colonies containing over 50 cells were counted up for analysis (g). h Cell colonies were stained by Phalloidin-cy3 after 10 days of culture for assessment of actin filaments containment and cellular protrusions (Scale bar=50 µm) (*P<0.05; **P=0.01; ***P<0.001; ****P<0.0001) {KD}

Immunogen image

PHF14 Polyclonal Antibody

Immunogen Image PHF14 Polyclonal Antibody

产品信息

PA5-72775

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

-

酶联免疫吸附实验 (ELISA)

Assay-Dependent

-

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

PHF14 antibody for a 19 amino acid peptide near the amino terminus of human PHF14. The immunogen is within amino acids 210 - 260 of PHF14.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Ambient (domestic); Wet ice (international)

靶标信息

PHF14 gene ontology annotations related to this gene include metal ion binding.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: unnamed protein product

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基因别名: 1110001C23Rik; 4932409F11Rik; 5730446A07Rik; mKIAA0783; RGD1563764

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UniProt ID: (Human) O94880, (Mouse) Q9D4H9

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Entrez Gene ID: (Human) 9678, (Rat) 500030, (Mouse) 75725

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protein binding zinc ion binding histone binding histone reader activity

negative regulation of transcription from RNA polymerase II promoter germinal center B cell differentiation chromatin organization chromatin remodeling regulation of transcription from RNA polymerase II promoter negative regulation of cell proliferation lung alveolus development negative regulation of mesenchymal cell proliferation negative regulation of platelet-derived growth factor receptor-alpha signaling pathway negative regulation of mesenchymal cell proliferation involved in lung development