Invitrogen

PHF14 Polyclonal Antibody

Name: PHF14 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-72775

高级验证

1 篇参考文献

查看其他4个PHF14抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)
高级验证 (1)

PHF14 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

PHF14 Antibody (PA5-72775) in ICC/IF

Immunofluorescence of PHF14 in 293 cells with PHF14 Polyclonal Antibody (Product # PA5-72775) at 5 µg/mL.

Product Image Gallery

PHF14 Antibody — PHF14 silencing in glioma cell lines decelerates proliferation and clonogenic growth. a, b The knockdown efficacy of PHF14 examined in U251, U87MG and A172 cells by qRT-PCR (a) and Western blot (b). c, d Cellular DNA replication is mitigated after PHF14 knocked down. Ten thousand U87MG, U251 and A172 cells per well were seeded in 96-well plates overnight. EdU and Hoechest co-staining was performed on the second day to assess cellular DNA replication activities (c) (Scale bar=100 µm). Values are counted as EdU+ cells/total cells (d). e-g PHF14 silenced cells showed weaker proliferation and colony formation potential. 500 U251 and A172 cells per well were cultured in 6-well plates in Complete growth medium for 10 days and then stained by 1% crystal violet for colony formation assay (e) and single colony morphology (f) (Scale bar=200 µm). The number of colonies containing over 50 cells were counted up for analysis (g). h Cell colonies were stained by Phalloidin-cy3 after 10 days of culture for assessment of actin filaments containment and cellular protrusions (Scale bar=50 µm) (*P<0.05; **P=0.01; ***P<0.001; ****P<0.0001) {KD}

Immunogen image

Immunogen Image PHF14 Polyclonal Antibody

产品信息

PA5-72775

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

PHF14 antibody for a 19 amino acid peptide near the amino terminus of human PHF14. The immunogen is within amino acids 210 - 260 of PHF14.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2718629

靶标信息

PHF14 gene ontology annotations related to this gene include metal ion binding.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: PHD finger protein 14

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基因别名: 1110001C23Rik; 4932409F11Rik; 5730446A07Rik; AA623952; AV297001; KIAA0783; mKIAA0783; PHF14; RGD1563764

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UniProt ID: (Human) O94880, (Mouse) Q9D4H9

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Entrez Gene ID: (Human) 9678, (Mouse) 75725, (Rat) 500030

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功能

zinc ion binding molecular_function metal ion binding DNA-binding transcription factor ubiquitin-protein ligase zinc finger transcription factor protein modifying enzyme gene-specific transcriptional regulator

参与通路

negative regulation of transcription from RNA polymerase II promoter lung alveolus development negative regulation of platelet-derived growth factor receptor-alpha signaling pathway negative regulation of mesenchymal cell proliferation involved in lung development negative regulation of cell proliferation negative regulation of mesenchymal cell proliferation

PHF14 Polyclonal Antibody

PHF14 Antibody (PA5-72775) in ICC/IF

产品信息

PA5-72775

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

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