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Invitrogen
PIAS2 Recombinant Rabbit Monoclonal Antibody (4I6K6)
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PIAS2 Antibody (MA5-35673) in WB
Western blot analysis of PIAS2 in extracts of various cell lines. Samples were incubated with PIAS2 Monoclonal antibody (Product # MA5-35673) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-35673
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
4I6K6
抗原
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PIAS2 (NP_0046622)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
3 mg/mL
规格
300 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: SPAVQIKIRE LYRRRYPRTL EGLSDLSTIK SSVFSLDGGS SPVEPDLAVA GIHSLPSTSV TPHSPSSPVG SVLLQDTKPT FEMQQPSPPI PPVHPDVQLK N
靶标信息
This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: androgen receptor-interacting protein 3; DAB2-interacting protein; E3 SUMO-protein transferase PIAS2; MGC102682; msx-interacting zinc finger protein; Msx-interacting-zinc finger; msx-interacting-zinc finger protein; protein inhibitor of activated STAT x; RING-type E3 ubiquitin transferase PIAS2; unnamed protein product
基因别名: ARIP3; Dib; DIP; Miz1; PiasX; PIASxalpha; PIASxalpha6; PIASxb; PIASxbeta; SIZ2
UniProt ID: (Mouse) Q8C5D8, (Rat) Q6AZ28
Entrez Gene ID: (Mouse) 17344, (Rat) 83422
DNA binding
transcription cofactor activity
transcription corepressor activity
protein binding
zinc ion binding
SUMO transferase activity
protein domain specific binding
estrogen receptor binding
ubiquitin protein ligase binding
glucocorticoid receptor binding
androgen receptor binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
ubiquitin protein ligase activity
SUMO ligase activity
transcription regulator inhibitor activity
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein sumoylation
androgen receptor signaling pathway
endoplasmic reticulum unfolded protein response
regulation of osteoblast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JAK-STAT cascade
positive regulation of dendrite morphogenesis
regulation of androgen receptor signaling pathway
negative regulation of androgen receptor signaling pathway
transforming growth factor beta receptor signaling pathway
spermatogenesis
response to testosterone
SMAD protein signal transduction
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