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Invitrogen
PIASy Polyclonal Antibody
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FIGURE: 1 / 3
PIASy Antibody (PA5-30628) in ICC/IF
PIASy Polyclonal Antibody detects PIAS4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PIAS4 protein stained by PIASy Polyclonal Antibody (Product # PA5-30628) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-30628
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human PIASy. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.94 mg/mL
Amount
94 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: human PIAS4-transfected 293T.
Predicted reactivity: Chimpanzee (97%), Bovine (87%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: e3 sumo- ligase pias4; E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PIAS-gamma; PIASy; polymorphic variant; PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; protein inhibitor of activated STAT protein PIASy; RING-type E3 ubiquitin transferase PIAS4; unnamed protein product; zinc finger, MIZ-type containing 6
Gene Aliases: PIAS-gamma; PIAS4; PIASG; PIASY; ZMIZ6
UniProt ID: (Human) Q8N2W9
Entrez Gene ID: (Human) 51588
negative regulation of transcription from RNA polymerase II promoter
hair follicle development
double-strand break repair
regulation of transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
central nervous system development
regulation of gene expression
negative regulation of macromolecule biosynthetic process
negative regulation of tumor necrosis factor-mediated signaling pathway
Wnt signaling pathway
protein sumoylation
positive regulation of protein sumoylation
vitamin D metabolic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
regulation of mRNA stability
negative regulation of transcription, DNA-templated
limb epidermis development
mRNA destabilization
regulation of cellular response to stress
negative regulation of protein localization to chromatin
positive regulation of keratinocyte apoptotic process
positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage
positive regulation of double-strand break repair via homologous recombination
negative regulation of double-strand break repair via homologous recombination
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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