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Invitrogen
PIN1 Recombinant Rabbit Monoclonal Antibody (PSH10-81)
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图: 1 / 10
PIN1 Antibody (MA570134) in ICC/IF
Immunocytochemistry analysis of PIN1 in NIH/3T3 cells. Samples were incubated with PIN1 monoclonal antibody (Product # MA570134) using a dilution of 1:100 in 1% BSA in PBST overnight at 4°C followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. PBS instead of the primary antibody wa... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA570134
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH10-81
抗原
Recombinant protein within human Pin1 aa 1-163.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Positive control: HeLa cell lysate, MCF7 cell lysate, Huh7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse liver tissue, mouse pancreas tissue, rat liver tissue, rat pancreas tissue, human pancreas tissue.
Subcellular location: Nucleus, Nucleus speckle, Cytoplasm.
靶标信息
PIN1 (Peptidylprolyl Cis/Trans Isomerase, NIMA-Interacting 1) is a Protein Coding gene. Diseases associated with PIN1 include Meibomian Cyst and Viral Exanthem. Among its related pathways are Transport of the SLBP independent Mature mRNA and Cytokine Signaling in Immune system. Gene Ontology (GO) annotations related to this gene include peptidyl-prolyl cis-trans isomerase activity and mitogen-activated protein kinase binding.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ARABIDOPSIS THALIANA PIN-FORMED 1; ATPIN1; EC 5.2.1.8; F6D5.2; F6D5_2; NIMA-interacting; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; PIN-FORMED 1; PPIase Pin1; prolyl isomerase; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; Rotamase Pin1
基因别名: 0610025L01Rik; D9Bwg1161e; PIN1
UniProt ID: (Mouse) Q9QUR7
Entrez Gene ID: (Mouse) 23988, (Rat) 298696
protein peptidyl-prolyl isomerization
positive regulation of protein phosphorylation
cell cycle
negative regulation of transforming growth factor beta receptor signaling pathway
regulation of cytokinesis
positive regulation of protein dephosphorylation
regulation of cell proliferation
negative regulation of neuron apoptotic process
positive regulation of neuron apoptotic process
positive regulation of GTPase activity
positive regulation of ubiquitin-protein transferase activity
regulation of pathway-restricted SMAD protein phosphorylation
positive regulation of cell growth involved in cardiac muscle cell development
negative regulation of ERK1 and ERK2 cascade
negative regulation of cell motility
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