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Agrisera
PIP1-1, PIP1-2, PIP1-3, PIP1-4, PIP1-5/Aquaporins Polyclonal Antibody
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PIP1-1, PIP1-2, PIP1-3, PIP1-4, PIP1-5/Aquaporins Antibody (AS22-4816) in WB
40-50 µg/well of total protein extracted freshly from Arabidopsis thaliana roots. Exact buffer components were: 50 m M Tris-HCl p H 8,20 m M EDTA, 1 m M DTT, 1% PIC and 400 m M Sucrose and denatured with 3 X sample buffer (62.5 m M Tris p H-6.8, 10% glycerin, 2% SDS, 5% Mercaptoethanol, 0,05% Bromophenol blue) at 70°C/5 min. Samples were separated in the cold on 8,5% SDS-PAGE and blotted for 1 h nitrocellul... View More
产品信息
AS22-4816
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from N terminus of Raphanus sativus.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
PIP1-1, PIP1-2, PIP1-3, PIP1-4 and PIP1-5 encode closely related plasma membrane intrinsic protein (PIP1) aquaporins that localize predominantly to the plant cell plasma membrane, where they form homotetrameric water-channel complexes that increase membrane water permeability and thereby support cell-to-cell water transport and whole-plant hydraulics. Structurally, PIP1 proteins are small integral membrane proteins of the major intrinsic protein family (approximately 280-290 amino acids) with the canonical aquaporin fold: six transmembrane helices plus two conserved half-helices that meet in the membrane to create a single pore per monomer; the proteins also contain the signature NPA motifs and an aromatic/arginine selectivity region typical of water-selective aquaporins. Functionally, genetic analyses in Arabidopsis indicate that PIP1 isoforms contribute measurably to tissue hydraulic conductivity (for example, loss of PIP1-2 reduces root hydrostatic hydraulic conductivity), and PIP1-1 has been implicated in coordinating water transport with nutrient-related developmental plasticity, including modulation of lateral root development and nitrate accumulation under low-nitrate conditions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: A_IG005I10.2; A_IG005I10_2; ATHH2; ATPIP1; F22L4.16; F22L4_16; F4I18.6; F5I10.2; F5I10_2; PIP1; PIP1;1; PIP1;2; PIP1;3; PIP1;4; PIP1A; PIP1B; PIP1C; PIP1E; PLASMA MEMBRANE INTRINSIC PROTEIN 1; PLASMA MEMBRANE INTRINSIC PROTEIN 1;1; TMP-A; TMP-B; TMP-C; TRANSMEMBRANE PROTEIN A; TRANSMEMBRANE PROTEIN C
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