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Invitrogen
PKC delta Recombinant Rabbit Monoclonal Antibody (JJ093-02)
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PKC delta Antibody (MA5-32482) in ICC/IF
Immunofluorescence analysis of PRKCD was performed using 70% confluent log phase HEK 293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PKC delta Recombinant Rabbit Monoclonal Antibody (JJ093-02) (Product # MA5-32482) at 1:100 dilution in 0.1% BS... View More
产品信息
MA5-32482
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JJ093-02
抗原
Recombinant protein within mouse PKC delta aa 525-671
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
The PKC family of serine/threonine kinases, including PRKCD (PKC delta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 1-May; kinase nPKC-delta; KPCD; nPKC-delta; Protein kinase C delta type; protein kinase C delta variant IX; protein kinase C delta VIII; protein kinase C, delta; protein kinase C, delta IV; protein kinase C, delta V; protein kinase C[d]; Tyrosine-protein kinase PRKCD
基因别名: AI385711; ALPS3; CVID9; D14Ertd420e; MAY1; nPKC-delta; PKC[d]; PKCD; PKCdelta; PRKCD
UniProt ID: (Human) Q05655, (Mouse) P28867, (Rat) P09215
Entrez Gene ID: (Human) 5580, (Mouse) 18753, (Rat) 170538
protein kinase activity
protein serine/threonine kinase activity
protein kinase C activity
calcium-independent protein kinase C activity
non-membrane spanning protein tyrosine kinase activity
protein binding
ATP binding
enzyme activator activity
enzyme binding
kinase binding
protein kinase binding
insulin receptor substrate binding
metal ion binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
TIR domain binding
non-receptor serine/threonine protein kinase
stimulatory C-type lectin receptor signaling pathway
protein phosphorylation
apoptotic process
cellular component disassembly involved in execution phase of apoptosis
cell cycle
signal transduction
activation of phospholipase C activity
intrinsic apoptotic signaling pathway in response to oxidative stress
regulation of receptor activity
immunoglobulin mediated immune response
histone phosphorylation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
peptidyl-tyrosine phosphorylation
termination of signal transduction
platelet activation
negative regulation of actin filament polymerization
positive regulation of endodeoxyribonuclease activity
negative regulation of protein binding
activation of protein kinase activity
interleukin-10 production
interleukin-12 production
positive regulation of superoxide anion generation
regulation of actin cytoskeleton organization
negative regulation of glial cell apoptotic process
positive regulation of protein dephosphorylation
Fc-gamma receptor signaling pathway involved in phagocytosis
B cell proliferation
neutrophil activation
positive regulation of protein import into nucleus
defense response to bacterium
negative regulation of MAP kinase activity
regulation of mRNA stability
negative regulation of insulin receptor signaling pathway
negative regulation of inflammatory response
negative regulation of peptidyl-tyrosine phosphorylation
protein stabilization
negative regulation of filopodium assembly
cell chemotaxis
interferon-gamma-mediated signaling pathway
cellular response to hydrogen peroxide
cellular response to hydroperoxide
negative regulation of platelet aggregation
cellular senescence
positive regulation of phospholipid scramblase activity
cellular response to angiotensin
positive regulation of ceramide biosynthetic process
positive regulation of glucosylceramide catabolic process
positive regulation of sphingomyelin catabolic process
positive regulation of response to DNA damage stimulus
positive regulation of apoptotic signaling pathway
response to oxidative stress
aspartate transport
phosphorylation
collagen metabolic process
cellular response to oxidative stress
intracellular signal transduction
regulation of phosphorylation
positive regulation of apoptotic process
positive regulation of MAP kinase activity
positive regulation of MAPK cascade
positive regulation of glucose import
protein autophosphorylation
response to hypoxia
aging
response to heat
response to mechanical stimulus
response to glucose
response to organonitrogen compound
response to organic cyclic compound
cellular response to insulin stimulus
cellular response to glucose starvation
response to drug
response to hydrogen peroxide
positive regulation of catalytic activity
response to amino acid
response to ethanol
