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Invitrogen
PKC epsilon Polyclonal Antibody
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FIGURE: 1 / 2
PKC epsilon Antibody (PA5-102580) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded PKC epsilon in rat heart tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with PKC epsilon polyclonal antibody (Product # PA5-102580) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-102580
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PRKCE(Accession Q02156), corresponding to amino acid residues P683-E733.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total PKC epsilon.
Target Information
The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MGC125656; MGC125657; nPKC-epsilon; PKC e; Protein kinase C epsilon type; protein kinase C, epsilon
Gene Aliases: 5830406C15Rik; nPKC-epsilon; PKC[e]; PKCE; Pkcea; PKCepsilon; PRKCE; R75156
UniProt ID: (Human) Q02156, (Mouse) P16054, (Rat) P09216
Entrez Gene ID: (Human) 5581, (Mouse) 18754, (Rat) 29340
actin monomer binding
protein serine/threonine kinase activity
protein kinase C activity
calcium-independent protein kinase C activity
signal transducer activity
protein binding
ATP binding
enzyme activator activity
enzyme binding
receptor activator activity
ethanol binding
metal ion binding
14-3-3 protein binding
nucleotide binding
protein kinase activity
receptor binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
SH3 domain binding
protein kinase binding
non-receptor serine/threonine protein kinase
protein modifying enzyme
macrophage activation involved in immune response
protein phosphorylation
apoptotic process
cell cycle
cell adhesion
signal transduction
activation of phospholipase C activity
positive regulation of epithelial cell migration
positive regulation of fibroblast migration
positive regulation of cell-substrate adhesion
peptidyl-serine phosphorylation
platelet activation
positive regulation of actin filament polymerization
negative regulation of protein ubiquitination
lipopolysaccharide-mediated signaling pathway
positive regulation of insulin secretion
positive regulation of synaptic transmission, GABAergic
positive regulation of cytokinesis
intracellular signal transduction
locomotory exploration behavior
TRAM-dependent toll-like receptor 4 signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to morphine
positive regulation of MAPK cascade
regulation of peptidyl-tyrosine phosphorylation
positive regulation of lipid catabolic process
release of sequestered calcium ion into cytosol
regulation of release of sequestered calcium ion into cytosol
cell division
regulation of insulin secretion involved in cellular response to glucose stimulus
positive regulation of mucus secretion
cellular response to ethanol
cellular response to prostaglandin E stimulus
cellular response to hypoxia
positive regulation of wound healing
positive regulation of receptor activity
negative regulation of sodium ion transmembrane transporter activity
positive regulation of cellular glucuronidation
immune system process
chemosensory behavior
negative regulation of mitochondrial membrane potential
phosphorylation
regulation of lipid metabolic process
cellular response to platelet-derived growth factor stimulus
negative regulation of apoptotic process
positive regulation of catalytic activity
negative regulation of release of sequestered calcium ion into cytosol
negative regulation of mitochondrial calcium ion concentration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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