Invitrogen

PKC epsilon Recombinant Rabbit Monoclonal Antibody (ARC1270)

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抗体检测数据 (1)

PKC epsilon Antibody in Western Blot (WB)

PKC epsilon Antibody in Western Blot (WB)

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PKC epsilon Antibody (MA5-35617) in WB

Western blot analysis of PKC epsilon in extracts of Mouse lung. Samples were incubated with PKC epsilon Monoclonal antibody (Product # MA5-35617) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

PKC epsilon Antibody in Western Blot (WB)

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PKC epsilon Antibody in Western Blot (WB) — Western blot analysis of PKC epsilon in extracts of Mouse lung. Samples were incubated with PKC epsilon Monoclonal antibody (Product # MA5-35617) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

产品信息

MA5-35617

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

ARC1270

抗原

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human PKC epsilon (Q02156)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.94 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2849517

产品详细信息

Immunogen sequence: RSRKFDEPRS RFYAAEVTSA LMFLHQHGVI YRDLKLDNIL LDAEGHCKLA DFGMCKEGIL NGVTTTTFCG TPDYIAPEIL QELEYGPSVD WWALGVLMYE M

靶标信息

The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: MGC125656; MGC125657; nPKC-epsilon; PKC e; Protein kinase C epsilon type

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基因别名: 5830406C15Rik; PKC[e]; Pkce; Pkcea; PKCepsilon; Prkce; R75156

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UniProt ID: (Mouse) P16054

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Entrez Gene ID: (Mouse) 18754

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nucleotide binding actin monomer binding protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-independent protein kinase C activity receptor binding protein binding ATP binding enzyme activator activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups SH3 domain binding enzyme binding protein kinase binding receptor activator activity ethanol binding metal ion binding 14-3-3 protein binding non-receptor serine/threonine protein kinase

参与通路

macrophage activation involved in immune response immune system process protein phosphorylation cell cycle cell adhesion chemosensory behavior positive regulation of epithelial cell migration positive regulation of fibroblast migration positive regulation of cell-substrate adhesion negative regulation of mitochondrial membrane potential phosphorylation peptidyl-serine phosphorylation regulation of lipid metabolic process positive regulation of actin filament polymerization lipopolysaccharide-mediated signaling pathway positive regulation of insulin secretion positive regulation of synaptic transmission, GABAergic positive regulation of cytokinesis intracellular signal transduction locomotory exploration behavior TRAM-dependent toll-like receptor 4 signaling pathway cellular response to platelet-derived growth factor stimulus negative regulation of apoptotic process positive regulation of catalytic activity positive regulation of I-kappaB kinase/NF-kappaB signaling response to morphine positive regulation of MAPK cascade regulation of peptidyl-tyrosine phosphorylation positive regulation of lipid catabolic process release of sequestered calcium ion into cytosol regulation of release of sequestered calcium ion into cytosol negative regulation of release of sequestered calcium ion into cytosol cell division negative regulation of mitochondrial calcium ion concentration regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of mucus secretion cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of wound healing positive regulation of receptor activity positive regulation of cellular glucuronidation