Invitrogen

PKP3 Monoclonal Antibody (23E3/4)

Name: PKP3 Monoclonal Antibody (23E3\/4)
Brand: Invitrogen
SKU: MA1-06511

高级验证

4 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

高级验证 (1)

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PKP3 Antibody (MA1-06511)

High K8 levels in the PKP3 knockdown clones are due to a decrease in phosphorylation. A. Protein extracts from the vector control (pTU6) and PKP3 knockdown clones (S9 and S10) were resolved on SDSPAGE gels and Western blots performed with the indicated antibodies. B. S9 cells were transfected with either dsRed or the shRNA resistant dsRed PKP3 3.7R cDNA. 48 hours post transfection cells were stained with an... View More

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PKP3 Antibody — High K8 levels in the PKP3 knockdown clones are due to a decrease in phosphorylation. A. Protein extracts from the vector control (pTU6) and PKP3 knockdown clones (S9 and S10) were resolved on SDSPAGE gels and Western blots performed with the indicated antibodies. B. S9 cells were transfected with either dsRed or the shRNA resistant dsRed PKP3 3.7R cDNA. 48 hours post transfection cells were stained with antibodies to K8 (green) and visualized by confocal microscopy. Note that dsRed PKP3 3.7R localizes to the border as previously described (indicated by arrow) [28]. Original magnification is 630X with a 2X optical zoom. Scale bar 5 µm. C. A real time PCR analysis to determine the mRNA levels of K8 and K18 was performed on RNA isolated from the vector control and PKP3 knockdown clones. GAPDH was used as an internal control for normalization. The Ct values for all samples are shown on the Y-axis. D. Protein extracts from the PKP3 knockdown clones or the vector control were subjected to 2-dimensional gel electrophoresis and Western blots performed with antibodies to K8. E. Protein extracts from the vector control cells or the PKP3 knockdown clones were resolved on SDS-PAGE gels followed by Western blotting with antibodies to K8 or phosphospecific antibodies against S73 (α-S73), S431 (α-S431) and actin. F. HCT116 cells transfected with GFPK8 or GFPS73A or GFP S431A were resolved on two dimensional gels followed by Western blots with antibodies to K8. MW markers are indicate {KD}

Immunogen image

Immunogen Image PKP3 Monoclonal Antibody (23E3/4)

产品信息

MA1-06511

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:25-1:200

免疫组化（冰冻） (IHC (F))

1:25-1:200

免疫细胞化学 (ICC/IF)

1:25-1:200

免疫沉淀 (IP)

Assay-dependent

其他PubMed (Misc)

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产品规格

种属反应

Dog, Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

23E3/4

抗原

Synthetic peptide corresponding to residues F(570) T P Q S R R L R E L P L A A D A L T F(589) of human plakophilin-3.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

purified

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2283813

产品详细信息

MA1-06511 detects plakophilin-3 in human, mouse, canine cells.

MA1-06511 has sucessfully been used in Western blotting, immunoprecipitation, immunocytochemistry and immunohistochemistry procedures. By Western blot it detects a ~87 kDa band representing Plakophilin-3.

The MA1-06511 immunogen is a synthetic peptide corresponding to residues F(570) T P Q S R R L R E L P L A A D A L T F(589) of human plakophilin-3.

靶标信息

Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalization, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localization in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of ~87 kDa.

仅用于科研。不用于诊断过程。未经明确授权不得转售。