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Invitrogen
PLK1 Monoclonal Antibody (E.456.6)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
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PLK1 Antibody (MA5-15113) in WB
Western blot was performed using Anti-PLK1 Monoclonal Antibody (E.456.6) (Product # MA5-15113) and a 62kDa band corresponding to Serine/threonine-protein kinase PLK1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of SW480 (Lane 1), K-562 (Lane 2), MDA-MB-231 (Lane 3) and Hep G2 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). T... View More
产品信息
MA5-15113
产品规格
种属反应
Human,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
E.456.6
抗原
Synthetic peptide corresponding to residues near the carboxy terminus of human PLK1
偶联物
形式
Liquid
浓度
38 µg/mL
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
<0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_10980410
产品详细信息
It is not recommended to aliquot this antibody.
靶标信息
PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cell cycle regulated protein kinase; PLK 1; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase; Polo-like kinase 1; polo-like kinase homolog; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14
基因别名: PLK; PLK1; STPK13
UniProt ID: (Human) P53350, (Rat) Q62673
Entrez Gene ID: (Human) 5347, (Rat) 25515
mitotic sister chromatid segregation
G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
mitotic cytokinesis
cytokinesis
microtubule bundle formation
protein phosphorylation
sister chromatid cohesion
mitotic nuclear division
mitotic nuclear envelope disassembly
mitotic spindle assembly checkpoint
regulation of mitotic cell cycle
cell proliferation
positive regulation of peptidyl-threonine phosphorylation
female meiosis chromosome segregation
protein ubiquitination
peptidyl-serine phosphorylation
regulation of mitotic metaphase/anaphase transition
anaphase-promoting complex-dependent catabolic process
G2 DNA damage checkpoint
protein destabilization
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
regulation of protein binding
homologous chromosome segregation
establishment of protein localization
negative regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of proteolysis
centrosome organization
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein transferase activity
regulation of cell cycle
synaptonemal complex disassembly
protein localization to chromatin
positive regulation of protein localization to nucleus
regulation of mitotic spindle assembly
regulation of cell cycle G2/M phase transition
positive regulation of ubiquitin protein ligase activity
polar body extrusion after meiotic divisions
response to antibiotic
