Invitrogen

PLZF Monoclonal Antibody (9E12), PE-Cyanine7, eBioscience™

Name: PLZF Monoclonal Antibody (9E12), PE-Cyanine7, eBioscience\u2122
Brand: Invitrogen
SKU: 25-9322-82

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2 篇参考文献

查看其他20个PLZF抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (1)

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PLZF Antibody (25-9322-82) in Flow

LEFT: C57BL/6 thymocytes were surface stained with Anti-Mouse NK1.1 APC (Product # 17-5941-63) and Anti-Mouse TCR beta FITC (Product # 11-5961-82) followed by intracellular staining with 0.125 µg of Anti-Human/Mouse PLZF PE-Cyanine7 using the Foxp3/Transcription Factor Staining Buffer Set and protocol (Product # 00-5523-00). Viable, TCR beta+ cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis. RIGHT: Normal human peripheral blood cells were surface stained with Anti-Human CD56 (NCAM) FI... View More

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产品信息

25-9322-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

9E12

抗原

PLFZ peptide

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2637115

产品详细信息

This 9E12 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors. Expression of this transcriptional repressor in immune cells differs between mice and humans. In mice, PLZF is highly expressed in immature CD1d-resricted NKT2 and NKT1 cells, and a subset of gamma delta (Vg1.1+Vd6.3+) T cells. Studies have also demonstrated expression of PLZF in non-invariant CD1d-restricted T cells, as well as non-CD1d-restricted innate T cells. In humans, PLZF is expressed in NK cells, gamma delta T cells, as well as CD4 and CD8+ T cells. PLZF is also expressed in MR1-specific mucosal-associated invariant T cells, as well as in MHC Class II-restricted T cells that develop via a thymocyte-thymocyte interaction in humans. PLZF is expressed by innate lymphoid cells and its progenitor cells. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells, NK cell function, cellular quiescence, growth suppression, and maintenance of homeostatis. Finally, PLZF has been shown to inhibit gene expression induced by retinoic acid receptor.

This 9E12 antibody stains mouse thymocytes brighter than Mags.21F7.

This 9E12 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Green's luxoid; luxoid; promyelocytic leukaemia zinc finger; Promyelocytic leukemia zinc finger protein; Zinc finger and BTB domain-containing protein 16; Zinc finger protein 145; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein PLZF

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基因别名: AI467657; lu; PLZF; ZBTB16; Zfp145; ZNF145

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UniProt ID: (Human) Q05516

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Entrez Gene ID: (Human) 7704, (Mouse) 235320

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功能