Invitrogen

PLZF Monoclonal Antibody (Mags.21F7), Brilliant Violet™ 711, eBioscience™

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产品信息

407-9320-82

应用
建议稀释比
已发表文章

流式细胞分析 (Flow)

1.0 µg/test
-
产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

推荐的同型对照

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet™ 711, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

Mags.21F7

偶联物

Brilliant Violet™ 711 Brilliant Violet™ 711 Brilliant Violet™ 711

激发/发射光谱

406/711 nm 查看光谱 spectra

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_3093245

产品详细信息

Description
This Mags.21F7 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors.

Applications Tested
This Mags.21F7 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Blocking Buffers
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Light sensitivity
This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation
• Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Our internal testing suggests that Brilliant Violet™ 711 (BV711) is not compatible with methanol-based fixation.

Excitation: 407 nm; Emission: 713 nm; Laser: Violet Laser.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

靶标信息

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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生物信息学

蛋白别名: Green's luxoid; luxoid; promyelocytic leukaemia zinc finger; Promyelocytic leukemia zinc finger protein; Zinc finger and BTB domain-containing protein 16; Zinc finger protein 145; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein PLZF

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基因别名: AI467657; lu; PLZF; ZBTB16; Zfp145; ZNF145

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UniProt ID: (Human) Q05516

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Entrez Gene ID: (Human) 7704, (Mouse) 235320

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功能
RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding protein binding protein C-terminus binding protein domain specific binding identical protein binding protein homodimerization activity metal ion binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding C2H2 zinc finger transcription factor
参与通路
negative regulation of transcription from RNA polymerase II promoter mesonephros development transcription, DNA-templated apoptotic process central nervous system development negative regulation of cell proliferation embryonic pattern specification anterior/posterior pattern specification protein ubiquitination hemopoiesis myeloid cell differentiation positive regulation of chondrocyte differentiation protein localization to nucleus embryonic hindlimb morphogenesis forelimb morphogenesis embryonic digit morphogenesis positive regulation of apoptotic process positive regulation of fat cell differentiation negative regulation of myeloid cell differentiation positive regulation of ossification negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated male germ-line stem cell asymmetric division positive regulation of NK T cell differentiation cartilage development positive regulation of cartilage development skeletal system development regulation of transcription, DNA-templated transcription from RNA polymerase II promoter embryonic limb morphogenesis hindlimb morphogenesis
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