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Invitrogen
PMCA2 ATPase Polyclonal Antibody
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PMCA2 ATPase Antibody (PA1-915) in IHC (P)
Immunohistochemistry was performed on cancer biopsies of deparaffinized Human cervical carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit po... View More
产品信息
PA1-915
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Pig,
Rat
已发表种属
C. elegans,
Chicken,
Hamster,
Human,
Mouse,
Pig,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic Peptide: T(5) N S D F Y S K N Q R N E S S(19)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-915 detects calcium pump of the plasma membrane 2 (PMCA2 ATPase) from human and rat and porcine tissues and cells.
PA1-915 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~127 kDa protein and an ~133 kDa protein representing PMCA2a and PMCA2b ATPase, respectively, from rat brain microsomal fractions.
PA1-915 immunizing peptide corresponds to amino acid residues 5-19 from human PMCA2 ATPase protein. This sequence is completely conserved between human and rat PMCA2 ATPase.
靶标信息
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ATPase isoform 2 Na+K+ transporting beta polypeptide 2; ATPase isoform 2, Na+K+ transporting, beta polypeptide 2; ATPase, Ca++ transporting, plasma membrane 2; plasma membrane Ca(2+)-ATPase; plasma membrane Ca++-ATPase; plasma membrane Ca2+ pump 2; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 2; plasma membrane calcium pump; Plasma membrane calcium pump isoform 2; Plasma membrane calcium-transporting ATPase 2; PMCA2
基因别名: ATP2B2; PMCA2; PMCA2a; PMCA2i
UniProt ID: (Human) Q01814, (Rat) P11506
Entrez Gene ID: (Human) 491, (Pig) 100625418, (Rat) 24215
transport
calcium ion transport
organelle organization
lactation
sensory perception of sound
locomotory behavior
regulation of cell size
cerebellar Purkinje cell differentiation
cerebellar granule cell differentiation
neuron differentiation
ion transmembrane transport
locomotion
serotonin metabolic process
otolith mineralization
cGMP metabolic process
regulation of synaptic plasticity
synapse organization
neuromuscular process controlling balance
detection of mechanical stimulus involved in sensory perception of sound
regulation of cytosolic calcium ion concentration
positive regulation of calcium ion transport
auditory receptor cell stereocilium organization
calcium ion transmembrane transport
cochlea development
regulation of cardiac conduction
cell morphogenesis
neural retina development
cellular calcium ion homeostasis
brain development
metabolic process
cerebellum development
cerebellar Purkinje cell layer morphogenesis
inner ear morphogenesis
inner ear development
inner ear receptor cell differentiation
calcium ion export
