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Invitrogen
POFUT1 Polyclonal Antibody
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POFUT1 Antibody (PA5-97014) in WB
Western blot analysis of POFUT1 in extracts of various cell lines. Samples were incubated with POFUT1 Polyclonal antibody (Product # PA5-97014) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-97014
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 28-194 of human POFUT1 (NP_7584361)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.71 mg/mL
规格
71 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: SWDPAGYLLY CPCMGRFGNQ ADHFLGSLAF AKLLNRTLAV PPWIEYQHHK PPFTNLHVSY QKYFKLEPLQ AYHRVISLED FMEKLAPTHW PPEKRVAYCF EVAAQRSPDK KTCPMKEGNP FGPFWDQFHV SFNKSELFTG ISFSASYREQ WSQRRENHSC VTLLFPR; Positive Samples: HepG2, HL-60, Mouse heart, Mouse liver; Cellular Location: Endoplasmic reticulum
靶标信息
POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GDP-fucose protein O-fucosyltransferase 1; O-FucT-1; Peptide-O-fucosyltransferase 1; pofut1 {ECO:0000312|RGD:1303001}
基因别名: Fut12; mKIAA0180; O-FucT-1; Pofut1
UniProt ID: (Mouse) Q91ZW2, (Rat) Q6EV70
Entrez Gene ID: (Mouse) 140484, (Rat) 311551
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