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Invitrogen
POLH Polyclonal Antibody
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POLH Antibody (PA5-76055) in ICC/IF
Immunofluorescence analysis of POLH was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with POLH Rabbit Polyclonal Antibody (Product # PA5-76055) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More
产品信息
PA5-76055
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant full length Human POLH.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
靶标信息
The XPA gene encodes a zinc metalloprotein that preferentially binds to DNA damaged by UV radiation and chemical carcinogens and is required for the incision step during nucleotide excision repair The XPB and XPD genes encode DNA helicases involved in several DNA metabolic pathways, including DNA repair and transcription, and the XPG gene product is an endonuclease that cuts on the 3' side of a DNA lesion during nucleotide excision repair. Molecular defects in the XP variant (POL H) group maintain normal excision repair, yet they result in a substantial reduction in the ability to synthesize intact daughter DNA strands during DNA replication following DNA damage.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DNA polymerase eta; DNA-directed DNA polymerase eta; FLJ16395; FLJ21978; polymerase (DNA directed), eta; polymerase (DNA directed), eta (RAD 30 related); polymerase (DNA) eta; RAD30 homolog A; Xeroderma pigmentosum variant type protein; Xeroderma pigmentosum variant type protein homolog; XP V
基因别名: POLH; RAD30; RAD30A; XP-V; XPV
UniProt ID: (Human) Q9Y253, (Mouse) Q9JJN0
Entrez Gene ID: (Human) 5429, (Rat) 316235, (Mouse) 80905
DNA synthesis involved in DNA repair
DNA replication
DNA repair
regulation of DNA repair
pyrimidine dimer repair
response to UV-C
translesion synthesis
error-free translesion synthesis
cellular response to UV-C
postreplication repair
biological_process
DNA biosynthetic process
cellular response to DNA damage stimulus
