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Invitrogen
POLR2K Polyclonal Antibody
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FIGURE: 1 / 1
POLR2K Antibody (PA5-117367) in WB
Western blot analysis of POLR2K in SH-SY5Y whole cell and nuclear extracts (30 µg). Samples were separated with 15% SDS-PAGE and incubated in POLR2K polyclonal antibody (Product # PA5-117367) using a dilution of 1:1,000, followed by HRP-conjugated anti-rabbit IgG. Detection was performed with Trident ECL plus-Enhanced.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-117367
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human POLR2K. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.58 mg/mL
Amount
158 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Centrifuge briefly prior to opening.
Target Information
RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. POLR2K (polymerase (RNA) II (DNA directed) polypeptide K), also known as RPB12, RPABC4, RPB7.0, hRPB7.0, hsRPB10a, RPB10alpha or ABC10-alpha, is a 58 amino acid nuclear protein belonging to the archaeal rpoP/eukaryotic RPC10 RNA polymerase subunit family. The gene encoding POLR2K is located on human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ABC10-alpha; DNA-directed RNA polymerase II subunit K; DNA-directed RNA polymerases I, II, and III subunit RPABC4; RNA polymerase II 7.0 kDa subunit; RNA polymerases I, II, and III subunit ABC4; RPB10alpha; RPB7.0
Gene Aliases: POLR2K
Entrez Gene ID: (Human) 5440
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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