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Agrisera
PPD2/Protein PEAPOD 2 Polyclonal Antibody
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PPD2/Protein PEAPOD 2 Antibody (AS15-3107) in WB
Equal volumes of total proteins from E. coli purified with GST agarose beads or N. benthamiana were extracted and denatured with LaemLli buffer (50 m M Tris p H 8,10 m M EDTA, 18% glycerol, and 10 m M DTT) at 95°C for 10 min. Samples were separated on 12% SDS-PAGE and blotted 30 min to Protran nitrocellulose membrane (Whatman) using semi-dry transfer. Blots were blocked with 0.25% BSA/0.25% gelatin for 60 m... View More
产品信息
AS15-3107
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana PPD2 sequence.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
PPD2 is a plant nuclear gene found in Arabidopsis thaliana within the PEAPOD locus, producing a plant-specific transcriptional regulator that functions as a DNA-associated repressor controlling lateral organ growth and morphology. The encoded PPD2 protein is characterized by a conserved TIFY family motif and a divergent CCT-like region consistent with a regulatory, non-enzymatic role, and genetic evidence supports functional redundancy with its paralog in restricting prolonged dispersed meristematic cell proliferation during leaf blade development. Loss of PPD2 activity contributes to enlarged, dome-shaped leaves and altered silique shape, consistent with a role in limiting lamina expansion and coordinating growth across the organ surface; mechanistically, PPD2 participates in transcriptional control of cell-cycle programs, including repression of CYCLIN D3 gene expression in concert with NINJA, thereby promoting timely arrest of cell division that is required for leaf flatness and appropriate organ size.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT4G14720; DL3400C; FCAALL.297; PEAPOD 2; TIFY4B
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