Search
Agrisera
PPDK/Pyruvate orthophosphate dikinase Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 1
PPDK/Pyruvate orthophosphate dikinase Antibody (AS13-2647) in WB
5 µg of total protein from samples such as Zea mays leaf, were extracted with Protein Extraction Buffer PEB (AS08 300). Samples were diluted with 1 X sample buffer (Nu PAGE LDS sample buffer (Invitrogen) supplemented with 50 m M DTT and heat at 70°C for 5 min and kept on ice before loading. Protein samples were separated on 4-12% Bolt Plus gels, LDS-PAGE and blotted for 70 minutes to PVDF using tank transfe... View More
产品信息
AS13-2647
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Purified recombinant enzyme consisting of residues 72-947 of Zea mays.
偶联物
形式
Lyophilized
规格
20 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana,Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus, Zea mays
靶标信息
PPDK encodes pyruvate, phosphate dikinase, a predominantly cytosolic enzyme in many organisms (and, in photosynthetic plants, also found in plastids of mesophyll cells) that functions as a reversible metabolic "energy-coupling" catalyst at the pyruvate/phosphoenolpyruvate node. The protein is a large, multi-domain phosphotransferase built from three major regions: an N-terminal nucleotide-binding domain that carries out the ATP/AMP partial reaction, a central phosphoryl-carrier (His) domain containing a conserved, transiently phosphorylated histidine essential for catalysis, and a C-terminal PEP/pyruvate-binding domain with an alpha/beta-barrel-type active-site architecture for binding pyruvate/PEP with divalent metal support. These reaction centers are spatially separated, and catalysis proceeds via a swiveling movement of the central His domain that shuttles the phosphoryl group between the N- and C-terminal active sites. Biochemically, PPDK catalyzes the interconversion of ATP + inorganic phosphate + pyruvate with AMP + pyrophosphate + phosphoenolpyruvate, enabling ATP-dependent PEP formation for carbon fixation support in C4 photosynthesis and gluconeogenic/PEP-supplying roles in diverse bacteria and protists, while also operating in the reverse direction in some microbes to support ATP formation depending on metabolic context.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: DL3805C; FCAALL.325; pyruvate orthophosphate dikinase
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
