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PPH1/TAP38/Protein phosphatase 1 Polyclonal Antibody
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PPH1/TAP38/Protein phosphatase 1 Antibody (AS16-4084) in WB
0.5-5 µg of chlorophyll per lane from Arabidopsis thaliana leaves WT (1-4) and delta-TAP 38 (5-8), ecotype Columbia were extracted according to Järvi et al 2016 (Plant Physiol 171:1,333-1,343) and denatured with SDS and 5% B-mercaptoethanol at 65°C for 10 min. After spinning down the proteins were separated on 12% mini-SDS-PAGE with 6 M urea and blotted 1 h to PVDF using semi-dry transfer (Hoefer). Membrane... View More
产品信息
AS16-4084
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana TAP38 sequence.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
PPH1 encodes a chloroplast-localized, thylakoid-associated serine/threonine protein phosphatase that resides predominantly in the stroma lamellae, positioning it to access antenna proteins that reversibly migrate between photosystems during acclimation to changing light. The protein is a monomeric PP2C-type phosphatase synthesized with an N-terminal chloroplast transit peptide and a conserved PP2C catalytic core that coordinates a binuclear divalent-metal center (Mn2+ or Mg2+) typical of PP2C enzymes; structural work further shows additional surface motifs that create substrate-recognition clefts enabling selective binding of phosphorylated N-terminal segments of LHCII components such as Lhcb1. Functionally, PPH1 is the principal phosphatase responsible for dephosphorylating major LHCII proteins (notably Lhcb1/Lhcb2) while showing limited activity toward photosystem II core phosphoproteins, thereby antagonizing the STN7 kinase pathway and promoting the transition from state 2 to state 1. Genetic loss of PPH1 leads to LHCII hyperphosphorylation, increased effective antenna association with photosystem I, and a pronounced defect in state transitions, underscoring its central role in balancing excitation energy distribution and maintaining photosynthetic redox homeostasis under fluctuating light.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: PROTEIN PHOSPHATASE 1; TAP38
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