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Invitrogen
PPM1F Polyclonal Antibody
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FIGURE: 1 / 4
PPM1F Antibody (PA5-112664) in ICC/IF
Immunocytochemichal analysis of PPM1F in HepG2 cells using a Polyclonal antibody (Product # PA5-112664) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
Product Details
PA5-112664
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Protein phosphatase 1F protein (1-76AA)
Conjugate
Form
Liquid
Concentration
4.07 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ca(2+)/calmodulin-dependent protein kinase phosphatase; calcium/calmodulin-dependent protein kinase phosphatase; CaM-kinase phosphatase; hFem-2; KIAA0015; Partner of PIX 2; partner of PIX2; PP2C phosphatase; Protein fem-2 homolog; Protein phosphatase 1F; protein phosphatase 1F (PP2C domain containing); protein phosphatase; POPX2; unnamed protein product
Gene Aliases: 1110021B16Rik; 4933427B07Rik; CAMKP; CaMKPase; FEM-2; hFEM-2; KIAA0015; mKIAA0015; POPX2; PPM1F
UniProt ID: (Human) P49593, (Mouse) Q8CGA0
Entrez Gene ID: (Human) 9647, (Mouse) 68606
phosphoprotein phosphatase activity
protein serine/threonine phosphatase activity
protein binding
protein tyrosine/serine/threonine phosphatase activity
hydrolase activity
calmodulin-dependent protein phosphatase activity
cation binding
metal ion binding
catalytic activity
phosphatase activity
protein phosphatase
apoptotic process
signal transduction
positive regulation of gene expression
positive regulation of epithelial cell migration
positive regulation of cell-substrate adhesion
positive regulation of cell migration
regulation of protein localization
intracellular signal transduction
negative regulation of transcription, DNA-templated
positive regulation of growth
positive regulation of chemotaxis
negative regulation of protein transport
positive regulation of stress fiber assembly
positive regulation of focal adhesion assembly
cellular response to xenobiotic stimulus
negative regulation of cell-cell adhesion mediated by cadherin
negative regulation of protein kinase activity
protein dephosphorylation
histone dephosphorylation
negative regulation of peptidyl-serine phosphorylation
cellular response to drug
peptidyl-threonine dephosphorylation
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of protein kinase activity by regulation of protein phosphorylation
intrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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