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Agrisera
PPR/Pentatricopeptide repeat-containing protein (chloroplastic) (SOT1) Polyclonal Antibody
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PPR/Pentatricopeptide repeat-containing protein (chloroplastic) (SOT1) Antibody (AS15-3102) in WB
Total protein (first 2 lanes to the left) or chloroplast protein (2 lanes to the right) of Arabidopsis thaliana were extracted with 100 m M Tris–HCl, p H 8.0,150 m M Na Cl, 1 m M EDTA, 0.5% Triton X-100,1 m M DTT, 1 m M PMSF, protease inhibitor cocktail, denatured with LaemmLi buffer (62.5 m M Tris–HCl p H 6.8,2% SDS, 10% glycerol, 100 m M DTT, 0.1% β-mercaptoethanol) at 95°C 5 min and separated on 8% SDS-P... View More
产品信息
AS15-3102
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana SOT 1 sequence.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
PPR (pentatricopeptide repeat) proteins are predominantly organelle-targeted, helical repeat RNA-binding factors that localize mainly to mitochondria (typically the matrix, with some members also reported in the nucleus) and act on post-transcriptional steps of gene expression. In mammals, a small set of nuclear-encoded PPR-motif proteins support mitochondrial transcript handling; the best-characterized example is LRPPRC, a leucine-rich protein containing multiple tandem PPR motifs that form an extended alpha-solenoid scaffold suited for sequence-selective RNA interactions and assembly of ribonucleoprotein complexes. Functionally, LRPPRC partners with the RNA-binding protein SLIRP to bind mitochondrial mRNAs cotranscriptionally, protect them from 3-prime exonucleolytic decay, promote appropriate 3-prime polyadenylation, and facilitate delivery and efficient translation of specific mitochondrial transcripts on the mitoribosome, thereby sustaining oxidative phosphorylation capacity (notably including effects on cytochrome c oxidase-related gene expression); disruption of this PPR-dependent mRNA stabilization/translation axis is a recognized mechanism underlying severe mitochondrial disease phenotypes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: F16P17.6; F16P17_6; Rf-like 9; RFL9
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