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Agrisera
PRK ribulose-5-P-kinase/Phosphoribulokinase Polyclonal Antibody
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PRK ribulose-5-P-kinase/Phosphoribulokinase Antibody (AS07-257) in WB
WT-Col-0 Arabidopsis thaliana leaves were frozen in liquid nitrogen and soluble proteins were extracted in a buffer containing 50 m M HEPES, 5 m M Na Cl and 10 m M Mg Cl 2.10 µg, 20 or 40 µg of separated in SDS-PAGE (15% acrylamide with 6 M urea) and blotted 1 h to a PVDF membrane using Höefer semi-dry blotter. Blot was blocked with 4% milk in TTBS for 1 h at room temperature (RT) with agitation. Blot was i... View More
产品信息
AS07-257
产品规格
种属反应
Algae,
Bacteria,
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from known PRK sequences including Arabidopsis thaliana.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Synechocystis sp. PCC6803, Synechococcus PCC 7942, Thalassiosira pseudonana, Oryza sativa, Zea mays
靶标信息
Phosphoribulokinase is a chloroplast enzyme in plants that regenerates ribulose 1,5 bisphosphate in the Calvin cycle by phosphorylating ribulose 5 phosphate using ATP. It is essential for sustaining carbon fixation and photosynthetic efficiency, and its activity is regulated by light via thioredoxin mediated redox control and by metabolite levels that reflect stromal energy status. Phosphoribulokinase forms part of the photosynthetic enzyme network coordinating flux through the Calvin cycle, and changes in its expression or activation influence growth, biomass accumulation, and tolerance to environmental stress. Mutations or reduced activity limit ribulose 1,5 bisphosphate supply, slow CO2 assimilation, and disrupt redox balance and carbohydrate partitioning.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT1G32060; PRK; T12O21.4; T12O21_4
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