Abnova

PRKCI Monoclonal Antibody (1G11)

Name: PRKCI Monoclonal Antibody (1G11)
Brand: Abnova
SKU: H00005584-M01

查看其他15个PKC lambda/iota抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

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PRKCI Antibody (H00005584-M01) in WB

Western Blot analysis of PRKCI expression in transfected 293T cell line by PRKCI monoclonal antibody (M01), clone 1G11. Lane 1: PRKCI transfected lysate(68.3 KDa). Lane 2: Non-transfected lysate. .

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产品信息

H00005584-M01

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

免疫细胞化学 (ICC/IF)

10-100 µg/mL

酶联免疫吸附实验 (ELISA)

10 µg/mL

功能检测 (Functional)

1-5 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

1G11

抗原

PRKCI (AAH22016, 1 a.a. approximately 587 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Product may be used with Western Blot (Transfected lysate).

Immunogen sequence: MSHTVAGGGS GDHSHQVRVK AYYRGDIMIT HFEPSISFEG LCNEVRDMCS FDNEQLFTMK WIDEEGDPCT VSSQLELEEA FRLYELNKDS ELLIHVFPCV PERPGMPCPG EDKSIYRRGA RRWRKLYCAN GHTFQAKRFN RRAHCAICTD RIWGLGRQGY KCINCKLLVH KKCHKLVTIE CGRHSLPQEP VMPMDQSSMH SDHAQTVIPY NPSSHESLDQ VGEEKEAMNT RESGKASSSL GLQDFDLLRV IGRGSYAKVL LVRLKKTDRI YAMKVVKKEL VNDDEDIDWV QTEKHVFEQA SNHPFLVGLH SCFQTESRLF FVIEYVNGGD LMFHMQRQRK LPEEHARFYS AEISLALNYL HERGIIYRDL KLDNVLLDSE GHIKLTDYGM CKEGLRPGDT TSTFCGTPNY IAPEILRGED YGFSVDWWAL GVLMFEMMAG RSPFDIVGSS DNPDQNTEDY LFQVILEKQI RIPRSMSVKA ASVLKSFLNK DPKERLGCLP QTGFADIQGH PFFRNVDWDM MEQKQVVPPF KPNISGEFGL DNFDSQFTNE RVQLTPDDDD IVRKIDQSEF EGFEYINPLL MSAEECV

靶标信息

The PKC family of serine/threonine kinases, including PRKCI (PKC iota), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.

仅用于科研。不用于诊断过程。未经明确授权不得转售。