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AA Sequence of recombinant protein: AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human EG-VEGF. Anti-Human EG-VEGF-specific antibody was purified by affinity chromatography employing an immobilized Human EG-VEGF matrix.
Sandwich ELISA: To detect Human EG-VEGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human EG-VEGF (500-P188GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human EG-VEGF.
Western Blot: To detect Human EG-VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EG-VEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Endocrine gland-derived vascular endothelial growth factor induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. Its expression is induced by hypoxia and is restricted to the steroidogenic glands. Its expression is often complementary to the expression of VEGF, suggesting that these molecules function in a coordinated manner.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: black mamba toxin-related protein; EG-VEGF; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; Prokineticin-1
基因别名: EGVEGF; PK1; PRK1; PROK1; UNQ600/PRO1186
UniProt ID: (Human) P58294
Entrez Gene ID: (Human) 84432