Invitrogen

PRSSL1 Polyclonal Antibody

问题与答案

问题与答案

问题与答案

抗体检测数据 (4)

PRSSL1 Antibody in Immunocytochemistry (ICC/IF)

PRSSL1 Antibody in Immunocytochemistry (ICC/IF)

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PRSSL1 Antibody (PA5-49256) in ICC/IF

Immunocytochemistry analysis of PRSSL1 in A549 cells. Samples were incubated with PRSSL1 polyclonal antibody (Product # PA5-49256) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-Rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized. Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:500 dilution (red). The nuclear counter stain is DAPI (blue).

PRSSL1 Antibody in Immunocytochemistry (ICC/IF)

PRSSL1 Antibody in Western Blot (WB)

PRSSL1 Antibody in Western Blot (WB)

PRSSL1 Antibody in Flow Cytometry (Flow)

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PRSSL1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of PRSSL1 in A549 cells. Samples were incubated with PRSSL1 polyclonal antibody (Product # PA5-49256) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-Rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized. Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:500 dilution (red). The nuclear counter stain is DAPI (blue).

PRSSL1 Antibody in Western Blot (WB) — Western blot analysis of PRSSL1 in human spleen lysate. Samples were incubated with PRSSL1 polyclonal antibody (Product # PA5-49256) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 30 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

PRSSL1 Antibody in Western Blot (WB) — Western blot analysis of PRSSL1 in human spleen lysate. Samples were incubated with PRSSL1 polyclonal antibody (Product # PA5-49256) using a dilution of 1:4,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 30 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

PRSSL1 Antibody in Flow Cytometry (Flow) — Flow cytometry of (overlay histogram) of PRSSL1 in A549 cells (green line). Samples were incubated with PRSSL1 polyclonal antibody (Product # PA5-49256) using a dilution of 1:25 dilution for 60 min at 37°C followed by Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 dilution for 40 min at 37°C. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the primary antibody. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Immunogen image

PRSSL1 Polyclonal Antibody

Immunogen Image PRSSL1 Polyclonal Antibody

产品信息

PA5-49256

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫细胞化学 (ICC/IF)

1:25

-

流式细胞分析 (Flow)

1:25

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 225-254 amino acids from the C-terminal region of human PRSSL1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.27 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Predicted to react with mouse and rat based on sequence homology.

靶标信息

Serine protease that cleaves preferentially after Arg residues. Can also cleave after citrulline (deimidated arginine) and methylarginine residues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Neutrophil serine protease 4; NSP4; protease, serine-like 1; Serine protease 1-like protein 1; Serine protease 57

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基因别名: NSP4; PRSS57; PRSSL1; UNQ782; UNQ782/PRO1599

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UniProt ID: (Human) Q6UWY2

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Entrez Gene ID: (Human) 400668

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serine-type endopeptidase activity heparin binding serine-type peptidase activity protease serine protease protein modifying enzyme

proteolysis