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Agrisera
PSA3/Photosystem I Assembly 3 Polyclonal Antibody
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PSA3/Photosystem I Assembly 3 Antibody (AS15-2872) in WB
5 µg of total protein from Zea mays leaf seedling tissue was extracted with protein extraction buffer (100 m M Tris p H 7.5,10% glycerol, 1 m M EDTA, 1 m M EGTA, 2 m M p MSF, 0.002 mg leupeptin, 0.002 mg pepstatin A, 2.5 m M DTT) and denatured in 1 XPSB (33 m M Tris p H 6.8,50 m M 2-Mercaptoethanol, 2% SDS, 10% glycerol, 0.1% bromophenol blue) at 70°C for 5 min, separated on 4-15% Tris-Glycine SDS-PAGE and ... View More
产品信息
AS15-2872
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant PSA3, amino acids 110 to 269 derived from Zea mays.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Zea mays
靶标信息
PSMA3 encodes a ubiquitously expressed, soluble proteasome alpha-type subunit that localizes predominantly to the cytosol and nucleus as a core constituent of the 20S proteasome (and, by extension, the 26S proteasome). The gene produces alternatively spliced transcripts that yield distinct protein isoforms, and the canonical product is a ~28 kDa, ~255 amino-acid member of the peptidase T1A family that adopts the conserved proteasome alpha-subunit fold and assembles into the outer heptameric alpha rings of the 20S core particle, where alpha-subunit N-termini contribute to formation of the gated entry pore controlling substrate access to the proteolytic chamber. Functionally, PSMA3 supports ubiquitin/ATP-dependent protein turnover by helping build and maintain the architecture of the 20S core that partners with regulatory particles to degrade polyubiquitinated substrates, thereby shaping proteostasis, cell-cycle progression, and stress responses; consistent with this role, PSMA3 has also been reported to bind diverse cellular proteins, linking the proteasome to broader regulatory pathways including aspects of mRNA metabolism.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT3G55250; chloroplastic; Photosystem I assembly factor PSA3; Photosystem I Assembly3
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