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Agrisera
PSB33/Rieske (2Fe-2S) domain-containing protein Polyclonal Antibody
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PSB33/Rieske (2Fe-2S) domain-containing protein Antibody (AS12-1852) in WB
Specified µg/µL of chlorophyll from Arabidopsis thaliana leaf extracted with sample buffer (2% SDS, 8% sucrose, 0.2 m M EDTA, 10 m M Tris HCl (p H 6.8) 4% beta-mercaptoethanol) were separated on 15% SDS-PAGE and blotted 1 h to PVDF. Blots were blocked with 10% milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:4 000 overnight at 4°C with agita... View More
产品信息
AS12-1852
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Part of Arabidopsis thaliana recombinant TEF5 protein, corresponding to epitopes 61-242.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Oryza sativa
靶标信息
PSB33 encodes a nucleus-derived chloroplast protein that is imported via an N-terminal transit peptide and accumulates as an integral component of the thylakoid membrane, where it associates with photosystem II (PSII)-light-harvesting complex II (LHCII) assemblies. In Arabidopsis, PSB33 is a small membrane protein (approximately 23 kDa by immunodetection) predicted to contain a single transmembrane helix that harbors an LHC-binding motif, plus a comparatively large surface-exposed head domain positioned to interact with PSII/LHCII components; biochemical fractionation and complex analyses place it near LHCII and the PSII core antenna subunit CP43. Functionally, PSB33 acts as an auxiliary PSII core factor that supports the stability and/or organization of PSII-LHCII supercomplexes and helps optimize photosynthetic performance under changing or stressful light conditions: loss-of-function mutants show reduced PSII-LHCII supercomplex accumulation, strongly impaired state transitions, reduced nonphotochemical quenching capacity, and heightened photosensitivity (including under UV-A), consistent with a role in maintaining a functional PSII pool during light stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT1G71500; F26A9.12; Light-harvesting-like 8; LIL8; PhotoSystem B protein 33
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