Invitrogen

PSMA5 Polyclonal Antibody

Name: PSMA5 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-1962

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6 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)
已发表图片 (3)

PSMA5 Antibody in Immunocytochemistry (ICC/IF)

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PSMA5 Antibody (PA1-1962) in ICC/IF

Immunofluorescence analysis of Proteasome 20S Subunit alpha-5 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSMA5 Rabbit Polyclonal (Product # PA1-1962) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj... View More

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Immunogen image

Immunogen Image PSMA5 Polyclonal Antibody

产品信息

PA1-1962

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

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免疫细胞化学 (ICC/IF)

2 µg/mL

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产品规格

种属反应

Hamster, Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues C A(120) L Q F G E E D A D P G A M(133) of human proteasome 20S subunit alpha 5.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2171717

产品详细信息

PA1-1962 detects proteasome 20S subunit alpha 5 from human, rat, mouse and hamster cell lysates.

PA1-1962 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a 26.5 kDa protein representing proteasome 20S subunit alpha 5 from human, rat, mouse, and hamster cell extracts.

PA1-1962 immunogen is a synthetic peptide corresponding to residues C A(120) L Q F G E E D A D P G A M(133) of human proteasome 20S subunit alpha 5. PA1-1962 immunizing peptide (Cat. # PEP-210) is available for use in neutralization and control experiments.

靶标信息

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: alpha-5; epididymis tissue sperm binding protein Li 11n; macropain subunit zeta; Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; proteasome (prosome, macropain) subunit, alpha type, 5; proteasome alpha 5 subunit; proteasome component 5; Proteasome subunit alpha type-5; proteasome subunit alpha type-5-like protein; Proteasome subunit alpha-5; proteasome subunit zeta; Proteasome zeta chain

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基因别名: PSC5; PSMA5; ZETA

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UniProt ID: (Human) P28066, (Mouse) Q9Z2U1