Invitrogen

PSMB5 Polyclonal Antibody

Name: PSMB5 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-977

高级验证

6个已发表图片

16 篇参考文献

查看其他8个PSMB5抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

PSMB5 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

PSMB5 Antibody (PA1-977) in ICC/IF

Immunofluorescence analysis of PSMB5 was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PSMB5 Polyclonal Antibody (Product # PA1-977) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More

Product Image Gallery

PSMB5 Antibody — Figure 5 ( a ) Immunoblots of proteasome catalytic subunits in H727 cells transfected with siRNA targeting beta5, beta2 or beta5i. ( b ) The catalytic activity of beta5 subunit was decreased in H727 cells transfected with siRNA targeting beta5 compared with H727 cells transfected with scrambled siRNA. ( c ) Effects of siRNA knockdown of beta5, beta5i, or beta2 on Cfz sensitivity (IC 50 values) in H23 and H727 cells. Data are shown as mean +- SD derived from a single non-linear regression based on n = 3 replicates per compound per concentration. ( d ) Heat map showing proteasome catalytic subunit activity profiles of 6 PI-naive patient MM samples purchased from Conversant Biologics and AllCells (left). The numbers represent hydrolysis rates of respective substrates (RFU/min, mean values derived from three technical replicates) and were converted to color format and clustered by using the program "R" ( http://www.R-project.org ) (left panel). Carfilzomib (Cfz) cell viability of the same 6 patient MM cells was measured via CellTiter-Glo Luminescent Cell Viability Assay (right panel). {KD}

Immunogen image

Immunogen Image PSMB5 Polyclonal Antibody

产品信息

PA1-977

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

查看12篇已发表文章 12篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:10-1:200

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Bovine, Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: C W(243) I R V S S D N V A D L H D K Y S(259)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2172052

产品详细信息

PA1-977 detects proteasome 20S X from bovine, mouse and human samples.

PA1-977 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~23 kDa protein representing proteasome 20S X.

The PA1-977 immunizing peptide corresponds to amino acid residues 243-259 from mouse proteasome 20S X. This sequence is 94% conserved in human and rat. PA1-977 immunizing peptide (Cat. # PEP-172) is available for use in neutralization and control procedures.

靶标信息

Cyclic nucleotide phosphodiesterases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: beta-5; Macropain epsilon chain; MGC118075; Multicatalytic endopeptidase complex epsilon chain; proteasome (prosome, macropain) subunit, beta type, 5; proteasome beta 5 subunit; proteasome beta type subunit 5; proteasome catalytic subunit 3; Proteasome chain 6; Proteasome epsilon chain; proteasome subunit beta 5; Proteasome subunit beta type-5; Proteasome subunit beta-5; Proteasome subunit MB1; Proteasome subunit X; proteasome subunit, beta type, 5; PSX large multifunctional protease X; testicular tissue protein Li 153

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基因别名: LMPX; MB1; PSMB5; X

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UniProt ID: (Human) P28074, (Bovine) Q32KL2, (Mouse) O55234

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Entrez Gene ID: (Human) 5693, (Bovine) 534640, (Mouse) 19173

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功能

threonine-type endopeptidase activity protein binding peptidase activity endopeptidase activity hydrolase activity protease

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