Invitrogen

PSMD8 Polyclonal Antibody

Name: PSMD8 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-144028

查看其他5个PSMD8抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (10)

PSMD8 Antibody in Immunocytochemistry (ICC/IF)

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PSMD8 Antibody (PA5-144028) in ICC/IF

Immunocytochemistry analysis of PSMD8 in A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in PSMD8 Polyclonal antibody (Product # PA5-144028) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More

PSMD8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image PSMD8 Polyclonal Antibody

产品信息

PA5-144028

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human PSMD8 recombinant protein (Position: A86-V350).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3075242

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human MCF-7 whole cell, human A549 whole cell. IHC: mouse brain tissue, rat brain tissue, human breast cancer tissue, human lienal pupture tissue, human lung cancer tissue, human tonsil tissue, human renal cancer tissue, human liver tissue. ICC/IF: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 26S proteasome non-ATPase regulatory subunit 8; 26S proteasome regulatory subunit p31; 26S proteasome regulatory subunit RPN12; 26S proteasome regulatory subunit S14; epididymis secretory sperm binding protein Li 91n; p31; proteasome (prosome, macropain) 26S subunit, non-ATPase, 8; proteasome 26S non-ATPase subunit 8; proteasome 26S subunit, non-ATPase, 8

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基因别名: 6720456J22Rik; AA407360; AL033291; AL033322; AL033323; C76433; HEL-S-91n; HIP6; HYPF; Nin1p; p31; PSMD8; Rpn12; S14

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UniProt ID: (Human) P48556, (Mouse) Q9CX56

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Entrez Gene ID: (Human) 5714, (Mouse) 57296, (Rat) 292766

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功能

molecular_function protease

参与通路