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PSMD9 Antibody (PA5-103514) in ICC/IF
Immunocytochemistry analysis of PSMD9 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PSMD9 polyclonal antibody (Product # PA5-103514) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-103514
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PSMD9(Accession O00233), corresponding to amino acid residues V23-R73.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2852850
产品详细信息
Antibody detects endogenous levels of total PSMD9.
靶标信息
Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 26S proteasome non-ATPase regulatory subunit 9; 26S proteasome regulatory subunit p27; homolog of rat Bridge 1; MGC8644; proteasome (prosome, macropain) 26S subunit, non-ATPase, 9; proteasome 26S non-ATPase regulatory subunit 9; Transactivating protein Bridge-1
基因别名: 1500011J20Rik; 2610202L11Rik; Bridge; Bridge-1; P27; PSMD9; Rpn4
UniProt ID: (Human) O00233, (Mouse) Q9CR00, (Rat) Q9WTV5
Entrez Gene ID: (Human) 5715, (Mouse) 67151, (Rat) 161475
MAPK cascade
protein polyubiquitination
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
ubiquitin-dependent protein catabolic process
regulation of cellular amino acid metabolic process
anaphase-promoting complex-dependent catabolic process
positive regulation of insulin secretion
tumor necrosis factor-mediated signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of mRNA stability
positive regulation of transcription, DNA-templated
negative regulation of insulin secretion
T cell receptor signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
Wnt signaling pathway, planar cell polarity pathway
proteasome regulatory particle assembly
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
positive regulation of transcription from RNA polymerase II promoter
type B pancreatic cell apoptotic process
